1tt9
From Proteopedia
(New page: 200px<br /><applet load="1tt9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tt9, resolution 3.42Å" /> '''Structure of the bif...) |
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| - | [[Image:1tt9.gif|left|200px]]<br /><applet load="1tt9" size=" | + | [[Image:1tt9.gif|left|200px]]<br /><applet load="1tt9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tt9, resolution 3.42Å" /> | caption="1tt9, resolution 3.42Å" /> | ||
'''Structure of the bifunctional and Golgi associated formiminotransferase cyclodeaminase octamer'''<br /> | '''Structure of the bifunctional and Golgi associated formiminotransferase cyclodeaminase octamer'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Mammalian formiminotransferase cyclodeaminase (FTCD), a 0.5 million Dalton | + | Mammalian formiminotransferase cyclodeaminase (FTCD), a 0.5 million Dalton homo-octameric enzyme, plays important roles in coupling histidine catabolism with folate metabolism and integrating the Golgi complex with the vimentin intermediate filament cytoskeleton. It is also linked to two human diseases, autoimmune hepatitis and glutamate formiminotransferase deficiency. Determination of the FTCD structure by X-ray crystallography and electron cryomicroscopy revealed that the eight subunits, each composed of distinct FT and CD domains, are arranged like a square doughnut. A key finding indicates that coupling of three subunits governs the octamer-dependent sequential enzyme activities, including channeling of intermediate and conformational change. The structure further shed light on the molecular nature of two strong antigenic determinants of FTCD recognized by autoantibodies from patients with autoimmune hepatitis and on the binding of thin vimentin filaments to the FTCD octamer. |
==About this Structure== | ==About this Structure== | ||
| - | 1TT9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | + | 1TT9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TT9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chen, D | + | [[Category: Chen, D H.]] |
[[Category: Chiu, W.]] | [[Category: Chiu, W.]] | ||
| - | [[Category: Ludtke, S | + | [[Category: Ludtke, S J.]] |
[[Category: Mao, Y.]] | [[Category: Mao, Y.]] | ||
| - | [[Category: Quiocho, F | + | [[Category: Quiocho, F A.]] |
| - | [[Category: Vyas, M | + | [[Category: Vyas, M N.]] |
| - | [[Category: Vyas, N | + | [[Category: Vyas, N K.]] |
[[Category: hepatitis autoantigen]] | [[Category: hepatitis autoantigen]] | ||
[[Category: intermediate channeling]] | [[Category: intermediate channeling]] | ||
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[[Category: vimentin]] | [[Category: vimentin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:17:03 2008'' |
Revision as of 13:17, 21 February 2008
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Structure of the bifunctional and Golgi associated formiminotransferase cyclodeaminase octamer
Overview
Mammalian formiminotransferase cyclodeaminase (FTCD), a 0.5 million Dalton homo-octameric enzyme, plays important roles in coupling histidine catabolism with folate metabolism and integrating the Golgi complex with the vimentin intermediate filament cytoskeleton. It is also linked to two human diseases, autoimmune hepatitis and glutamate formiminotransferase deficiency. Determination of the FTCD structure by X-ray crystallography and electron cryomicroscopy revealed that the eight subunits, each composed of distinct FT and CD domains, are arranged like a square doughnut. A key finding indicates that coupling of three subunits governs the octamer-dependent sequential enzyme activities, including channeling of intermediate and conformational change. The structure further shed light on the molecular nature of two strong antigenic determinants of FTCD recognized by autoantibodies from patients with autoimmune hepatitis and on the binding of thin vimentin filaments to the FTCD octamer.
About this Structure
1TT9 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure of the bifunctional and Golgi-associated formiminotransferase cyclodeaminase octamer., Mao Y, Vyas NK, Vyas MN, Chen DH, Ludtke SJ, Chiu W, Quiocho FA, EMBO J. 2004 Aug 4;23(15):2963-71. Epub 2004 Jul 22. PMID:15272307
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