1tut
From Proteopedia
(New page: 200px<br /><applet load="1tut" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tut" /> '''J4/5 Loop from the Candida albicans and Cand...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1tut.gif|left|200px]]<br /><applet load="1tut" size=" | + | [[Image:1tut.gif|left|200px]]<br /><applet load="1tut" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tut" /> | caption="1tut" /> | ||
'''J4/5 Loop from the Candida albicans and Candida dubliniensis Group I Introns'''<br /> | '''J4/5 Loop from the Candida albicans and Candida dubliniensis Group I Introns'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The J4/5 loop of group I introns has tertiary interactions with the P1 | + | The J4/5 loop of group I introns has tertiary interactions with the P1 helix that position the P1 substrate for the self-splicing reaction. The J4/5 loop of Candida albicans and Candida dubliniensis, 5'GAAGG3'/3'UAAUU5', potentially contains two A.A pairs flanked by one G.U pair on one side and two G.U pairs on the other side. Results from optical melting, nuclear magnetic resonance spectroscopy, and functional group substitution experiments with a mimic of the C. albicans and C. dubliniensis J4/5 loop are consistent with the adenosines forming tandem sheared A.A pairs with a cross-strand stack and only the G.U pair not adjacent to an A.A pair forming a static wobble G.U pair. The two G.U pairs adjacent to the tandem A.A pairs are likely in a dynamic equilibrium between multiple conformations. Although Co(NH(3))(6)(3+) stabilizes the loop by several kilocalories per mole at 37 degrees C, addition of Mg(2+) or Co(NH(3))(6)(3+) has no effect on the structure of the loop. The tandem G.U pairs provide a pocket of negative charge for Co(NH(3))(6)(3+) to bind. The results contribute to understanding the structure and dynamics of purine-rich internal loops and potential G.U pairs adjacent to internal loops. |
==About this Structure== | ==About this Structure== | ||
| - | 1TUT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1TUT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TUT OCA]. |
==Reference== | ==Reference== | ||
Structural features and thermodynamics of the J4/5 loop from the Candida albicans and Candida dubliniensis group I introns., Znosko BM, Kennedy SD, Wille PC, Krugh TR, Turner DH, Biochemistry. 2004 Dec 21;43(50):15822-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15595837 15595837] | Structural features and thermodynamics of the J4/5 loop from the Candida albicans and Candida dubliniensis group I introns., Znosko BM, Kennedy SD, Wille PC, Krugh TR, Turner DH, Biochemistry. 2004 Dec 21;43(50):15822-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15595837 15595837] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Kennedy, S | + | [[Category: Kennedy, S D.]] |
| - | [[Category: Krugh, T | + | [[Category: Krugh, T R.]] |
| - | [[Category: Turner, D | + | [[Category: Turner, D H.]] |
| - | [[Category: Wille, P | + | [[Category: Wille, P C.]] |
| - | [[Category: Znosko, B | + | [[Category: Znosko, B M.]] |
[[Category: aa pairs]] | [[Category: aa pairs]] | ||
[[Category: gu pairs]] | [[Category: gu pairs]] | ||
| Line 24: | Line 24: | ||
[[Category: tandem mismatch]] | [[Category: tandem mismatch]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:17:33 2008'' |
Revision as of 13:17, 21 February 2008
|
J4/5 Loop from the Candida albicans and Candida dubliniensis Group I Introns
Overview
The J4/5 loop of group I introns has tertiary interactions with the P1 helix that position the P1 substrate for the self-splicing reaction. The J4/5 loop of Candida albicans and Candida dubliniensis, 5'GAAGG3'/3'UAAUU5', potentially contains two A.A pairs flanked by one G.U pair on one side and two G.U pairs on the other side. Results from optical melting, nuclear magnetic resonance spectroscopy, and functional group substitution experiments with a mimic of the C. albicans and C. dubliniensis J4/5 loop are consistent with the adenosines forming tandem sheared A.A pairs with a cross-strand stack and only the G.U pair not adjacent to an A.A pair forming a static wobble G.U pair. The two G.U pairs adjacent to the tandem A.A pairs are likely in a dynamic equilibrium between multiple conformations. Although Co(NH(3))(6)(3+) stabilizes the loop by several kilocalories per mole at 37 degrees C, addition of Mg(2+) or Co(NH(3))(6)(3+) has no effect on the structure of the loop. The tandem G.U pairs provide a pocket of negative charge for Co(NH(3))(6)(3+) to bind. The results contribute to understanding the structure and dynamics of purine-rich internal loops and potential G.U pairs adjacent to internal loops.
About this Structure
1TUT is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural features and thermodynamics of the J4/5 loop from the Candida albicans and Candida dubliniensis group I introns., Znosko BM, Kennedy SD, Wille PC, Krugh TR, Turner DH, Biochemistry. 2004 Dec 21;43(50):15822-37. PMID:15595837
Page seeded by OCA on Thu Feb 21 15:17:33 2008
Categories: Protein complex | Kennedy, S D. | Krugh, T R. | Turner, D H. | Wille, P C. | Znosko, B M. | Aa pairs | Gu pairs | Internal loop | Nmr | Rna | Tandem mismatch
