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1tv3
From Proteopedia
(New page: 200px<br /><applet load="1tv3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tv3, resolution 2.20Å" /> '''Crystal structure of...) |
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| - | [[Image:1tv3.jpg|left|200px]]<br /><applet load="1tv3" size=" | + | [[Image:1tv3.jpg|left|200px]]<br /><applet load="1tv3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tv3, resolution 2.20Å" /> | caption="1tv3, resolution 2.20Å" /> | ||
'''Crystal structure of the N-methyl-hydroxylamine MtmB complex'''<br /> | '''Crystal structure of the N-methyl-hydroxylamine MtmB complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously | + | L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1TV3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri] with BG4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1TV3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri] with <scene name='pdbligand=BG4:'>BG4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TV3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanosarcina barkeri]] | [[Category: Methanosarcina barkeri]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chan, M | + | [[Category: Chan, M K.]] |
| - | [[Category: Ferguson, T | + | [[Category: Ferguson, T K.]] |
[[Category: Gallucci, J.]] | [[Category: Gallucci, J.]] | ||
[[Category: Hao, B.]] | [[Category: Hao, B.]] | ||
| - | [[Category: Kang, P | + | [[Category: Kang, P T.]] |
| - | [[Category: Krzycki, J | + | [[Category: Krzycki, J A.]] |
| - | [[Category: Soares, J | + | [[Category: Soares, J A.]] |
[[Category: Zhao, G.]] | [[Category: Zhao, G.]] | ||
[[Category: BG4]] | [[Category: BG4]] | ||
[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:17:43 2008'' |
Revision as of 13:17, 21 February 2008
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Crystal structure of the N-methyl-hydroxylamine MtmB complex
Overview
L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.
About this Structure
1TV3 is a Single protein structure of sequence from Methanosarcina barkeri with as ligand. Full crystallographic information is available from OCA.
Reference
Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid., Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki JA, Chan MK, Chem Biol. 2004 Sep;11(9):1317-24. PMID:15380192
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