This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1twi
From Proteopedia
(New page: 200px<br /><applet load="1twi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1twi, resolution 2.00Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1twi.gif|left|200px]]<br /><applet load="1twi" size=" | + | [[Image:1twi.gif|left|200px]]<br /><applet load="1twi" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1twi, resolution 2.00Å" /> | caption="1twi, resolution 2.00Å" /> | ||
'''Crystal structure of Diaminopimelate Decarboxylase from m. jannaschii in co-complex with L-lysine'''<br /> | '''Crystal structure of Diaminopimelate Decarboxylase from m. jannaschii in co-complex with L-lysine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cocrystal structures of Methanococcus jannaschii diaminopimelate | + | Cocrystal structures of Methanococcus jannaschii diaminopimelate decarboxylase (DAPDC) bound to a substrate analog, azelaic acid, and its L-lysine product have been determined at 2.6 A and 2.0 A, respectively. This PLP-dependent enzyme is responsible for the final step of L-lysine biosynthesis in bacteria and plays a role in beta-lactam antibiotic resistance in Staphylococcus aureus. Substrate specificity derives from recognition of the L-chiral center of diaminopimelate and a system of ionic "molecular rulers" that dictate substrate length. A coupled-enzyme assay system permitted measurement of kinetic parameters for recombinant DAPDCs and inhibition constants (K(i)) for azelaic acid (89 microM) and other substrate analogs. Implications for rational design of broad-spectrum antimicrobial agents targeted against DAPDCs of drug-resistant strains of bacterial pathogens, such as Staphylococcus aureus, are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1TWI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with MG, LYS and PLP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Diaminopimelate_decarboxylase Diaminopimelate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.20 4.1.1.20] Full crystallographic information is available from [http:// | + | 1TWI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=LYS:'>LYS</scene> and <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Diaminopimelate_decarboxylase Diaminopimelate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.20 4.1.1.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWI OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bonanno, J | + | [[Category: Bonanno, J B.]] |
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
[[Category: He, G.]] | [[Category: He, G.]] | ||
| - | [[Category: Lencastre, H | + | [[Category: Lencastre, H De.]] |
| - | [[Category: NYSGXRC, New | + | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] |
| - | [[Category: Pinho, M | + | [[Category: Pinho, M G.]] |
| - | [[Category: Rajashankar, K | + | [[Category: Rajashankar, K R.]] |
| - | [[Category: Ray, S | + | [[Category: Ray, S S.]] |
[[Category: Tomasz, A.]] | [[Category: Tomasz, A.]] | ||
[[Category: LYS]] | [[Category: LYS]] | ||
| Line 36: | Line 36: | ||
[[Category: t135]] | [[Category: t135]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:05 2008'' |
Revision as of 13:18, 21 February 2008
|
Crystal structure of Diaminopimelate Decarboxylase from m. jannaschii in co-complex with L-lysine
Overview
Cocrystal structures of Methanococcus jannaschii diaminopimelate decarboxylase (DAPDC) bound to a substrate analog, azelaic acid, and its L-lysine product have been determined at 2.6 A and 2.0 A, respectively. This PLP-dependent enzyme is responsible for the final step of L-lysine biosynthesis in bacteria and plays a role in beta-lactam antibiotic resistance in Staphylococcus aureus. Substrate specificity derives from recognition of the L-chiral center of diaminopimelate and a system of ionic "molecular rulers" that dictate substrate length. A coupled-enzyme assay system permitted measurement of kinetic parameters for recombinant DAPDCs and inhibition constants (K(i)) for azelaic acid (89 microM) and other substrate analogs. Implications for rational design of broad-spectrum antimicrobial agents targeted against DAPDCs of drug-resistant strains of bacterial pathogens, such as Staphylococcus aureus, are discussed.
About this Structure
1TWI is a Single protein structure of sequence from Methanocaldococcus jannaschii with , and as ligands. Active as Diaminopimelate decarboxylase, with EC number 4.1.1.20 Full crystallographic information is available from OCA.
Reference
Cocrystal structures of diaminopimelate decarboxylase: mechanism, evolution, and inhibition of an antibiotic resistance accessory factor., Ray SS, Bonanno JB, Rajashankar KR, Pinho MG, He G, De Lencastre H, Tomasz A, Burley SK, Structure. 2002 Nov;10(11):1499-508. PMID:12429091
Page seeded by OCA on Thu Feb 21 15:18:05 2008
Categories: Diaminopimelate decarboxylase | Methanocaldococcus jannaschii | Single protein | Bonanno, J B. | Burley, S K. | He, G. | Lencastre, H De. | NYSGXRC, New York Structural GenomiX Research Consortium. | Pinho, M G. | Rajashankar, K R. | Ray, S S. | Tomasz, A. | LYS | MG | PLP | Antibiotic resistance | Lysine biosynthesis | New york structural genomix research consortium | Nysgxrc | Protein structure initiative | Psi | Structural genomics | T135
