1txa
From Proteopedia
(New page: 200px<br /><applet load="1txa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1txa" /> '''SOLUTION NMR STRUCTURE OF TOXIN B, A LONG NE...) |
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| - | [[Image:1txa.jpg|left|200px]]<br /><applet load="1txa" size=" | + | [[Image:1txa.jpg|left|200px]]<br /><applet load="1txa" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1txa" /> | caption="1txa" /> | ||
'''SOLUTION NMR STRUCTURE OF TOXIN B, A LONG NEUROTOXIN FROM THE VENOM OF THE KING COBRA, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''SOLUTION NMR STRUCTURE OF TOXIN B, A LONG NEUROTOXIN FROM THE VENOM OF THE KING COBRA, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The solution structure of toxin b, a long neurotoxin (73 amino acids and 5 | + | The solution structure of toxin b, a long neurotoxin (73 amino acids and 5 disulfides) from the venom of Ophiophagus hannah (king cobra), has been determined using 1H NMR and dynamical simulated annealing techniques. The structures were calculated using 485 distance constraints and 52 dihedral angle restraints. The 21 structures that were obtained satisfy the experimental restraints and possess good nonbonded contacts. Analysis of the converged structures revealed that the protein consists of a core region from which three finger-like loops extend outwards. The regular secondary structure in toxin b includes a double and a triple stranded antiparallel beta sheet. Comparison with the solution structures of other long neurotoxins reveals that although the structure of toxin b is similar to those of previously reported long neurotoxins, clear local structural differences are observed in regions proposed to be involved in binding to the acetylcholine receptor. A positively charged cluster is found in the C-terminal tail, in Loop III, and in the tip of Loop II. This cationic cluster could be crucial for the binding of the long neurotoxins to the acetylcholine receptor. |
==About this Structure== | ==About this Structure== | ||
| - | 1TXA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ophiophagus_hannah Ophiophagus hannah]. Full crystallographic information is available from [http:// | + | 1TXA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ophiophagus_hannah Ophiophagus hannah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ophiophagus hannah]] | [[Category: Ophiophagus hannah]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chang, C | + | [[Category: Chang, C C.]] |
[[Category: Jayaraman, G.]] | [[Category: Jayaraman, G.]] | ||
| - | [[Category: Kumar, T | + | [[Category: Kumar, T K.S.]] |
| - | [[Category: Peng, S | + | [[Category: Peng, S S.]] |
[[Category: Yu, C.]] | [[Category: Yu, C.]] | ||
[[Category: multigene family]] | [[Category: multigene family]] | ||
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[[Category: venom]] | [[Category: venom]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:18 2008'' |
Revision as of 13:18, 21 February 2008
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SOLUTION NMR STRUCTURE OF TOXIN B, A LONG NEUROTOXIN FROM THE VENOM OF THE KING COBRA, MINIMIZED AVERAGE STRUCTURE
Overview
The solution structure of toxin b, a long neurotoxin (73 amino acids and 5 disulfides) from the venom of Ophiophagus hannah (king cobra), has been determined using 1H NMR and dynamical simulated annealing techniques. The structures were calculated using 485 distance constraints and 52 dihedral angle restraints. The 21 structures that were obtained satisfy the experimental restraints and possess good nonbonded contacts. Analysis of the converged structures revealed that the protein consists of a core region from which three finger-like loops extend outwards. The regular secondary structure in toxin b includes a double and a triple stranded antiparallel beta sheet. Comparison with the solution structures of other long neurotoxins reveals that although the structure of toxin b is similar to those of previously reported long neurotoxins, clear local structural differences are observed in regions proposed to be involved in binding to the acetylcholine receptor. A positively charged cluster is found in the C-terminal tail, in Loop III, and in the tip of Loop II. This cationic cluster could be crucial for the binding of the long neurotoxins to the acetylcholine receptor.
About this Structure
1TXA is a Single protein structure of sequence from Ophiophagus hannah. Full crystallographic information is available from OCA.
Reference
Solution structure of toxin b, a long neurotoxin from the venom of the king cobra (Ophiophagus hannah)., Peng SS, Kumar TK, Jayaraman G, Chang CC, Yu C, J Biol Chem. 1997 Mar 21;272(12):7817-23. PMID:9065446
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