1tz6
From Proteopedia
(New page: 200px<br /><applet load="1tz6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tz6, resolution 2.70Å" /> '''Crystal structure of...) |
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| - | [[Image:1tz6.gif|left|200px]]<br /><applet load="1tz6" size=" | + | [[Image:1tz6.gif|left|200px]]<br /><applet load="1tz6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tz6, resolution 2.70Å" /> | caption="1tz6, resolution 2.70Å" /> | ||
'''Crystal structure of aminoimidazole riboside kinase from Salmonella enterica complexed with aminoimidazole riboside and ATP analog'''<br /> | '''Crystal structure of aminoimidazole riboside kinase from Salmonella enterica complexed with aminoimidazole riboside and ATP analog'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structures of a Salmonella enterica aminoimidazole riboside | + | The crystal structures of a Salmonella enterica aminoimidazole riboside (AIRs) kinase, its complex with the substrate AIRs, and its complex with AIRs and an ATP analog were determined at 2.6 angstroms, 2.9 angstroms, and 2.7 angstroms, respectively. The product of the Salmonella-specific gene stm4066, AIRs kinase, is a homodimer with one active site per monomer. The core structure, consisting of an eight-stranded beta sheet flanked by eight alpha helices, indicates that AIRs kinase is a member of the ribokinase superfamily. Unlike ribokinase and adenosine kinase in this superfamily, AIRs kinase does not show significant conformational changes upon substrate binding. The active site is covered by a lid formed by residues 16-28 and 86-100. A comparison of the structure of AIRs kinase with other ribokinase superfamily members suggests that the active site lid and conformational changes that occur upon substrate binding may be advanced features in the evolution of the ribokinase superfamily. |
==About this Structure== | ==About this Structure== | ||
| - | 1TZ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2] with MG, K, ACP and AIS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Fructokinase Fructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.4 2.7.1.4] Full crystallographic information is available from [http:// | + | 1TZ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=ACP:'>ACP</scene> and <scene name='pdbligand=AIS:'>AIS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Fructokinase Fructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.4 2.7.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZ6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dougherty, M.]] | [[Category: Dougherty, M.]] | ||
| - | [[Category: Downs, D | + | [[Category: Downs, D M.]] |
| - | [[Category: Ealick, S | + | [[Category: Ealick, S E.]] |
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
[[Category: ACP]] | [[Category: ACP]] | ||
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[[Category: ribokinase fold]] | [[Category: ribokinase fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:55 2008'' |
Revision as of 13:18, 21 February 2008
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Crystal structure of aminoimidazole riboside kinase from Salmonella enterica complexed with aminoimidazole riboside and ATP analog
Overview
The crystal structures of a Salmonella enterica aminoimidazole riboside (AIRs) kinase, its complex with the substrate AIRs, and its complex with AIRs and an ATP analog were determined at 2.6 angstroms, 2.9 angstroms, and 2.7 angstroms, respectively. The product of the Salmonella-specific gene stm4066, AIRs kinase, is a homodimer with one active site per monomer. The core structure, consisting of an eight-stranded beta sheet flanked by eight alpha helices, indicates that AIRs kinase is a member of the ribokinase superfamily. Unlike ribokinase and adenosine kinase in this superfamily, AIRs kinase does not show significant conformational changes upon substrate binding. The active site is covered by a lid formed by residues 16-28 and 86-100. A comparison of the structure of AIRs kinase with other ribokinase superfamily members suggests that the active site lid and conformational changes that occur upon substrate binding may be advanced features in the evolution of the ribokinase superfamily.
About this Structure
1TZ6 is a Single protein structure of sequence from Salmonella typhimurium lt2 with , , and as ligands. Active as Fructokinase, with EC number 2.7.1.4 Full crystallographic information is available from OCA.
Reference
Crystal structure of an aminoimidazole riboside kinase from Salmonella enterica: implications for the evolution of the ribokinase superfamily., Zhang Y, Dougherty M, Downs DM, Ealick SE, Structure. 2004 Oct;12(10):1809-21. PMID:15458630
Page seeded by OCA on Thu Feb 21 15:18:55 2008
Categories: Fructokinase | Salmonella typhimurium lt2 | Single protein | Dougherty, M. | Downs, D M. | Ealick, S E. | Zhang, Y. | ACP | AIS | K | MG | Alpha/beta | Ribokinase fold
