1tzo
From Proteopedia
(New page: 200px<br /><applet load="1tzo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tzo, resolution 3.60Å" /> '''Crystal Structure of...) |
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| - | [[Image:1tzo.gif|left|200px]]<br /><applet load="1tzo" size=" | + | [[Image:1tzo.gif|left|200px]]<br /><applet load="1tzo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tzo, resolution 3.60Å" /> | caption="1tzo, resolution 3.60Å" /> | ||
'''Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore'''<br /> | '''Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore'''<br /> | ||
==Overview== | ==Overview== | ||
| - | After binding to cellular receptors and proteolytic activation, the | + | After binding to cellular receptors and proteolytic activation, the protective antigen component of anthrax toxin forms a heptameric prepore. The prepore later undergoes pH-dependent conversion to a pore, mediating translocation of the edema and lethal factors to the cytosol. We describe structures of the prepore (3.6 A) and a prepore:receptor complex (4.3 A) that reveal the location of pore-forming loops and an unexpected interaction of the receptor with the pore-forming domain. Lower pH is required for prepore-to-pore conversion in the presence of the receptor, indicating that this interaction regulates pH-dependent pore formation. We present an example of a receptor negatively regulating pH-dependent membrane insertion. |
==About this Structure== | ==About this Structure== | ||
| - | 1TZO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1TZO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bacillus anthracis]] | [[Category: Bacillus anthracis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Collier, R | + | [[Category: Collier, R J.]] |
| - | [[Category: Lacy, D | + | [[Category: Lacy, D B.]] |
| - | [[Category: Melnyk, R | + | [[Category: Melnyk, R A.]] |
| - | [[Category: Wigelsworth, D | + | [[Category: Wigelsworth, D J.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: heptamer]] | [[Category: heptamer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:05 2008'' |
Revision as of 13:19, 21 February 2008
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Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore
Overview
After binding to cellular receptors and proteolytic activation, the protective antigen component of anthrax toxin forms a heptameric prepore. The prepore later undergoes pH-dependent conversion to a pore, mediating translocation of the edema and lethal factors to the cytosol. We describe structures of the prepore (3.6 A) and a prepore:receptor complex (4.3 A) that reveal the location of pore-forming loops and an unexpected interaction of the receptor with the pore-forming domain. Lower pH is required for prepore-to-pore conversion in the presence of the receptor, indicating that this interaction regulates pH-dependent pore formation. We present an example of a receptor negatively regulating pH-dependent membrane insertion.
About this Structure
1TZO is a Single protein structure of sequence from Bacillus anthracis with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of heptameric protective antigen bound to an anthrax toxin receptor: a role for receptor in pH-dependent pore formation., Lacy DB, Wigelsworth DJ, Melnyk RA, Harrison SC, Collier RJ, Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13147-51. Epub 2004 Aug 23. PMID:15326297
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