1u0l

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1u0l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u0l, resolution 2.80&Aring;" /> '''Crystal structure of...)
Line 1: Line 1:
-
[[Image:1u0l.jpg|left|200px]]<br /><applet load="1u0l" size="450" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1u0l.jpg|left|200px]]<br /><applet load="1u0l" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1u0l, resolution 2.80&Aring;" />
caption="1u0l, resolution 2.80&Aring;" />
'''Crystal structure of YjeQ from Thermotoga maritima'''<br />
'''Crystal structure of YjeQ from Thermotoga maritima'''<br />
==Overview==
==Overview==
-
We have determined the crystal structure of the GDP complex of the YjeQ, protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPase, subfamaily. TmYjeQ, a homologue of Escherichia coli YjeQ, which is known, to bind to the ribosome, is composed of three domains: an N-terminal, oligonucleotide/oligosaccharide-binding fold domain, a central GTPase, domain, and a C-terminal zinc-finger domain. The crystal structure of, TmYjeQ reveals two interesting domains: a circularly permutated GTPase, domain and an unusual zinc-finger domain. The binding mode of GDP in the, GTPase domain of TmYjeQ is similar to those of GDP or GTP analogs in ras, proteins, a prototype GTPase. The N-terminal, oligonucleotide/oligosaccharide-binding fold domain, together with the, GTPase domain, forms the extended RNA-binding site. The C-terminal domain, has an unusual zinc-finger motif composed of Cys-250, Cys-255, Cys-263, and His-257, with a remote structural similarity to a portion of a, DNA-repair protein, rad51 fragment. The overall structural features of, TmYjeQ make it a good candidate for an RNA-binding protein, which is, consistent with the biochemical data of the YjeQ subfamily in binding to, the ribosome.
+
We have determined the crystal structure of the GDP complex of the YjeQ protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPase subfamaily. TmYjeQ, a homologue of Escherichia coli YjeQ, which is known to bind to the ribosome, is composed of three domains: an N-terminal oligonucleotide/oligosaccharide-binding fold domain, a central GTPase domain, and a C-terminal zinc-finger domain. The crystal structure of TmYjeQ reveals two interesting domains: a circularly permutated GTPase domain and an unusual zinc-finger domain. The binding mode of GDP in the GTPase domain of TmYjeQ is similar to those of GDP or GTP analogs in ras proteins, a prototype GTPase. The N-terminal oligonucleotide/oligosaccharide-binding fold domain, together with the GTPase domain, forms the extended RNA-binding site. The C-terminal domain has an unusual zinc-finger motif composed of Cys-250, Cys-255, Cys-263, and His-257, with a remote structural similarity to a portion of a DNA-repair protein, rad51 fragment. The overall structural features of TmYjeQ make it a good candidate for an RNA-binding protein, which is consistent with the biochemical data of the YjeQ subfamily in binding to the ribosome.
==About this Structure==
==About this Structure==
-
1U0L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with ZN and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U0L OCA].
+
1U0L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0L OCA].
==Reference==
==Reference==
Line 13: Line 13:
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
-
[[Category: BSGC, Berkeley.Structural.Genomics.Center.]]
+
[[Category: BSGC, Berkeley Structural Genomics Center.]]
[[Category: Jaru, J.]]
[[Category: Jaru, J.]]
[[Category: Kim, R.]]
[[Category: Kim, R.]]
-
[[Category: Kim, S.H.]]
+
[[Category: Kim, S H.]]
[[Category: Lou, Y.]]
[[Category: Lou, Y.]]
-
[[Category: Shin, D.H.]]
+
[[Category: Shin, D H.]]
[[Category: Yokota, H.]]
[[Category: Yokota, H.]]
[[Category: GDP]]
[[Category: GDP]]
Line 32: Line 32:
[[Category: zinc-finger]]
[[Category: zinc-finger]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:45:06 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:24 2008''

Revision as of 13:19, 21 February 2008


1u0l, resolution 2.80Å

Drag the structure with the mouse to rotate

Crystal structure of YjeQ from Thermotoga maritima

Overview

We have determined the crystal structure of the GDP complex of the YjeQ protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPase subfamaily. TmYjeQ, a homologue of Escherichia coli YjeQ, which is known to bind to the ribosome, is composed of three domains: an N-terminal oligonucleotide/oligosaccharide-binding fold domain, a central GTPase domain, and a C-terminal zinc-finger domain. The crystal structure of TmYjeQ reveals two interesting domains: a circularly permutated GTPase domain and an unusual zinc-finger domain. The binding mode of GDP in the GTPase domain of TmYjeQ is similar to those of GDP or GTP analogs in ras proteins, a prototype GTPase. The N-terminal oligonucleotide/oligosaccharide-binding fold domain, together with the GTPase domain, forms the extended RNA-binding site. The C-terminal domain has an unusual zinc-finger motif composed of Cys-250, Cys-255, Cys-263, and His-257, with a remote structural similarity to a portion of a DNA-repair protein, rad51 fragment. The overall structural features of TmYjeQ make it a good candidate for an RNA-binding protein, which is consistent with the biochemical data of the YjeQ subfamily in binding to the ribosome.

About this Structure

1U0L is a Single protein structure of sequence from Thermotoga maritima with and as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of YjeQ from Thermotoga maritima contains a circularly permuted GTPase domain., Shin DH, Lou Y, Jancarik J, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13198-203. Epub 2004 Aug 26. PMID:15331784

Page seeded by OCA on Thu Feb 21 15:19:24 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools