1gk8

From Proteopedia

Revision as of 13:09, 30 October 2007

RUBISCO FROM CHLAMYDOMONAS REINHARDTII

Overview

The crystal structure of Rubisco (ribulose 1,5-bisphosphate, carboxylase/oxygenase) from the unicellular green alga Chlamydomonas, reinhardtii has been determined to 1.4 A resolution. Overall, the, structure shows high similarity to the previously determined structures of, L8S8 Rubisco enzymes. The largest difference is found in the loop between, beta strands A and B of the small subunit (betaA-betaB loop), which is, longer by six amino acid residues than the corresponding region in Rubisco, from Spinacia. Mutations of residues in the betaA-betaB loop have been, shown to affect holoenzyme stability and catalytic properties. The, information contained in the Chlamydomonas structure enables a more, reliable analysis of the effect of these mutations. No electron density, was observed for the ... [(full description)]

About this Structure

1GK8 is a [Protein complex] structure of sequences from [Chlamydomonas reinhardtii] with MG, CAP and EDO as [ligands]. Active as [Ribulose-bisphosphate carboxylase], with EC number [4.1.1.39]. Structure known Active Site: MGA. Full crystallographic information is available from [OCA].

Reference

First crystal structure of Rubisco from a green alga, Chlamydomonas reinhardtii., Taylor TC, Backlund A, Bjorhall K, Spreitzer RJ, Andersson I, J Biol Chem. 2001 Dec 21;276(51):48159-64. Epub 2001 Oct 18. PMID:11641402

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