1u4c
From Proteopedia
(New page: 200px<br /><applet load="1u4c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u4c, resolution 2.35Å" /> '''Structure of spindle...) |
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| - | [[Image:1u4c.jpg|left|200px]]<br /><applet load="1u4c" size=" | + | [[Image:1u4c.jpg|left|200px]]<br /><applet load="1u4c" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u4c, resolution 2.35Å" /> | caption="1u4c, resolution 2.35Å" /> | ||
'''Structure of spindle checkpoint protein Bub3'''<br /> | '''Structure of spindle checkpoint protein Bub3'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Bub3 is one of at least six proteins that transmit the spindle assembly | + | Bub3 is one of at least six proteins that transmit the spindle assembly checkpoint signal. These proteins delay cell cycle progression from metaphase to anaphase in response to attachment defects between kinetochores and spindle microtubules and to tension defects between sister chromatids. To explore the molecular interactions mediated by Bub3, we have determined the crystal structure of the Saccharomyces cerevisiae protein Bub3p at 2.35 A resolution. Bub3p is a seven-blade beta-propeller, although its sequence diverges from that of other WD40 family members. Several loops are substantially elongated, but extra domains or insertions are not present at the termini. In particular, two extended loops project from the top face of the propeller, forming a cleft. Amino acid residues across the top face and one aspect of the lateral surface (spanning blades 5-6) are highly conserved among Bub3 proteins. We propose that these conserved surfaces are the loci for key interactions with conserved motifs in spindle checkpoint proteins Bub1 and Mad3/BubR1. Comparison of the Bub3 sequence to the WD40 protein, Rae1, shows high sequence conservation along the same surfaces. Rae1 interaction with Bub1 is, therefore, likely to involve a similar mode of binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1U4C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 1U4C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U4C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Harrison, S | + | [[Category: Harrison, S C.]] |
| - | [[Category: Larsen, N | + | [[Category: Larsen, N A.]] |
[[Category: beta propeller]] | [[Category: beta propeller]] | ||
[[Category: wd-40 protein]] | [[Category: wd-40 protein]] | ||
[[Category: wd40 protein]] | [[Category: wd40 protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:20:37 2008'' |
Revision as of 13:20, 21 February 2008
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Structure of spindle checkpoint protein Bub3
Overview
Bub3 is one of at least six proteins that transmit the spindle assembly checkpoint signal. These proteins delay cell cycle progression from metaphase to anaphase in response to attachment defects between kinetochores and spindle microtubules and to tension defects between sister chromatids. To explore the molecular interactions mediated by Bub3, we have determined the crystal structure of the Saccharomyces cerevisiae protein Bub3p at 2.35 A resolution. Bub3p is a seven-blade beta-propeller, although its sequence diverges from that of other WD40 family members. Several loops are substantially elongated, but extra domains or insertions are not present at the termini. In particular, two extended loops project from the top face of the propeller, forming a cleft. Amino acid residues across the top face and one aspect of the lateral surface (spanning blades 5-6) are highly conserved among Bub3 proteins. We propose that these conserved surfaces are the loci for key interactions with conserved motifs in spindle checkpoint proteins Bub1 and Mad3/BubR1. Comparison of the Bub3 sequence to the WD40 protein, Rae1, shows high sequence conservation along the same surfaces. Rae1 interaction with Bub1 is, therefore, likely to involve a similar mode of binding.
About this Structure
1U4C is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of the spindle assembly checkpoint protein Bub3., Larsen NA, Harrison SC, J Mol Biol. 2004 Dec 3;344(4):885-92. PMID:15544799
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