1u53
From Proteopedia
(New page: 200px<br /><applet load="1u53" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u53, resolution 1.56Å" /> '''Novel X-Ray Structur...) |
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| - | [[Image:1u53.gif|left|200px]]<br /><applet load="1u53" size=" | + | [[Image:1u53.gif|left|200px]]<br /><applet load="1u53" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u53, resolution 1.56Å" /> | caption="1u53, resolution 1.56Å" /> | ||
'''Novel X-Ray Structure of Na-ASP-2, a PR-1 protein from the nematode parasite Necator americanus and a vaccine antigen for human hookworm infection'''<br /> | '''Novel X-Ray Structure of Na-ASP-2, a PR-1 protein from the nematode parasite Necator americanus and a vaccine antigen for human hookworm infection'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Human hookworm infection is a major cause of anemia and malnutrition of | + | Human hookworm infection is a major cause of anemia and malnutrition of adults and children in the developing world. As part of on-going efforts to control hookworm infection, The Human Hookworm Vaccine Initiative has identified candidate vaccine antigens from the infective L3 larval stages of the parasite, including a family of pathogenesis-related (PR) proteins known as the Ancylostoma-secreted proteins (ASPs). A novel crystal structure of Na-ASP-2, a PR-1 protein secreted by infective larvae of the human hookworm Necator americanus, has been solved to resolution limits of 1.68 A and to an R-factor of 17% using the recombinant protein expressed in and secreted by Pichia pastoris. The overall fold of Na-ASP-2 is a three-layer alphabetaalpha sandwich flanked by an N-terminal loop and a short, cysteine-rich C terminus. Our structure reveals a large central cavity that is flanked by His129 and Glu106, two residues that are well conserved in all parasitic nematode L3 ASPs. Na-ASP-2 has structural and charge similarities to chemokines, which suggests that Na-ASP-2 may be an extra-cellular ligand of an unknown receptor. Na-ASP-2 is a useful homology model for NIF, a natural antagonistic ligand of CR3 receptor. From these modeling studies, possible binding modes were predicted. In addition, this first structure of a PR-1 protein from parasitic helminths may shed light on the molecular basis of host-parasite interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 1U53 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Necator_americanus Necator americanus]. Full crystallographic information is available from [http:// | + | 1U53 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Necator_americanus Necator americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U53 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Necator americanus]] | [[Category: Necator americanus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Asojo, O | + | [[Category: Asojo, O A.]] |
| - | [[Category: Borgstahl, G | + | [[Category: Borgstahl, G E.O.]] |
[[Category: Deumic, V.]] | [[Category: Deumic, V.]] | ||
[[Category: Dhar, K.]] | [[Category: Dhar, K.]] | ||
[[Category: Goud, G.]] | [[Category: Goud, G.]] | ||
| - | [[Category: Hotez, P | + | [[Category: Hotez, P J.]] |
[[Category: Liu, S.]] | [[Category: Liu, S.]] | ||
[[Category: Loukas, A.]] | [[Category: Loukas, A.]] | ||
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[[Category: antibiotic]] | [[Category: antibiotic]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:20:37 2008'' |
Revision as of 13:20, 21 February 2008
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Novel X-Ray Structure of Na-ASP-2, a PR-1 protein from the nematode parasite Necator americanus and a vaccine antigen for human hookworm infection
Overview
Human hookworm infection is a major cause of anemia and malnutrition of adults and children in the developing world. As part of on-going efforts to control hookworm infection, The Human Hookworm Vaccine Initiative has identified candidate vaccine antigens from the infective L3 larval stages of the parasite, including a family of pathogenesis-related (PR) proteins known as the Ancylostoma-secreted proteins (ASPs). A novel crystal structure of Na-ASP-2, a PR-1 protein secreted by infective larvae of the human hookworm Necator americanus, has been solved to resolution limits of 1.68 A and to an R-factor of 17% using the recombinant protein expressed in and secreted by Pichia pastoris. The overall fold of Na-ASP-2 is a three-layer alphabetaalpha sandwich flanked by an N-terminal loop and a short, cysteine-rich C terminus. Our structure reveals a large central cavity that is flanked by His129 and Glu106, two residues that are well conserved in all parasitic nematode L3 ASPs. Na-ASP-2 has structural and charge similarities to chemokines, which suggests that Na-ASP-2 may be an extra-cellular ligand of an unknown receptor. Na-ASP-2 is a useful homology model for NIF, a natural antagonistic ligand of CR3 receptor. From these modeling studies, possible binding modes were predicted. In addition, this first structure of a PR-1 protein from parasitic helminths may shed light on the molecular basis of host-parasite interactions.
About this Structure
1U53 is a Single protein structure of sequence from Necator americanus. Full crystallographic information is available from OCA.
Reference
X-ray structure of Na-ASP-2, a pathogenesis-related-1 protein from the nematode parasite, Necator americanus, and a vaccine antigen for human hookworm infection., Asojo OA, Goud G, Dhar K, Loukas A, Zhan B, Deumic V, Liu S, Borgstahl GE, Hotez PJ, J Mol Biol. 2005 Feb 25;346(3):801-14. Epub 2005 Jan 12. PMID:15713464
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