1u5w
From Proteopedia
(New page: 200px<br /><applet load="1u5w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u5w, resolution 2.3Å" /> '''Crystal structure of ...) |
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| - | [[Image:1u5w.gif|left|200px]]<br /><applet load="1u5w" size=" | + | [[Image:1u5w.gif|left|200px]]<br /><applet load="1u5w" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u5w, resolution 2.3Å" /> | caption="1u5w, resolution 2.3Å" /> | ||
'''Crystal structure of hypothetical protein yjjX from Escherichia coli'''<br /> | '''Crystal structure of hypothetical protein yjjX from Escherichia coli'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Inosine triphosphate (ITP) and xanthosine triphosphate (XTP) are formed | + | Inosine triphosphate (ITP) and xanthosine triphosphate (XTP) are formed upon deamination of ATP and GTP as a result of exposure to chemical mutagens and oxidative damage. Nucleic acid synthesis requires safeguard mechanisms to minimize undesired lethal incorporation of ITP and XTP. Here, we present the crystal structure of YjjX, a protein of hitherto unknown function. The three-dimensional fold of YjjX is similar to those of Mj0226 from Methanococcus janschii, which possesses nucleotidase activity, and of Maf from Bacillus subtilis, which can bind nucleotides. Biochemical analyses of YjjX revealed it to exhibit specific phosphatase activity for inosine and xanthosine triphosphates and have a possible interaction with elongation factor Tu. The enzymatic activity of YjjX as an inosine/xanthosine triphosphatase provides evidence for a plausible protection mechanism by clearing the noncanonical nucleotides from the cell during oxidative stress in E. coli. |
==About this Structure== | ==About this Structure== | ||
| - | 1U5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1U5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5W OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: BSGI, Montreal-Kingston | + | [[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]] |
[[Category: Jia, Z.]] | [[Category: Jia, Z.]] | ||
| - | [[Category: Singh, V | + | [[Category: Singh, V K.]] |
[[Category: Zheng, J.]] | [[Category: Zheng, J.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:20:54 2008'' |
Revision as of 13:20, 21 February 2008
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Crystal structure of hypothetical protein yjjX from Escherichia coli
Overview
Inosine triphosphate (ITP) and xanthosine triphosphate (XTP) are formed upon deamination of ATP and GTP as a result of exposure to chemical mutagens and oxidative damage. Nucleic acid synthesis requires safeguard mechanisms to minimize undesired lethal incorporation of ITP and XTP. Here, we present the crystal structure of YjjX, a protein of hitherto unknown function. The three-dimensional fold of YjjX is similar to those of Mj0226 from Methanococcus janschii, which possesses nucleotidase activity, and of Maf from Bacillus subtilis, which can bind nucleotides. Biochemical analyses of YjjX revealed it to exhibit specific phosphatase activity for inosine and xanthosine triphosphates and have a possible interaction with elongation factor Tu. The enzymatic activity of YjjX as an inosine/xanthosine triphosphatase provides evidence for a plausible protection mechanism by clearing the noncanonical nucleotides from the cell during oxidative stress in E. coli.
About this Structure
1U5W is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Identification of an ITPase/XTPase in Escherichia coli by structural and biochemical analysis., Zheng J, Singh VK, Jia Z, Structure. 2005 Oct;13(10):1511-20. PMID:16216582
Page seeded by OCA on Thu Feb 21 15:20:54 2008
