1u6h
From Proteopedia
(New page: 200px<br /><applet load="1u6h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u6h, resolution 2.38Å" /> '''Vinculin head (0-258...) |
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| - | [[Image:1u6h.gif|left|200px]]<br /><applet load="1u6h" size=" | + | [[Image:1u6h.gif|left|200px]]<br /><applet load="1u6h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u6h, resolution 2.38Å" /> | caption="1u6h, resolution 2.38Å" /> | ||
'''Vinculin head (0-258) in complex with the talin vinculin binding site 2 (849-879)'''<br /> | '''Vinculin head (0-258) in complex with the talin vinculin binding site 2 (849-879)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The cytoskeletal protein talin plays a key role in activating integrins | + | The cytoskeletal protein talin plays a key role in activating integrins and in coupling them to the actin cytoskeleton. Its N-terminal globular head, which binds beta integrins, is linked to an extended rod having a C-terminal actin binding site and several vinculin binding sites (VBSs). The NMR structure of residues 755-889 of the rod (containing a VBS) is shown to be an amphipathic four-helix bundle with a left-handed topology. A talin peptide corresponding to the VBS binds the vinculin head; the X-ray crystallographic structure of this complex shows that the residues which interact with vinculin are buried in the hydrophobic core of the talin fragment. NMR shows that the interaction involves a major structural change in the talin fragment, including unfolding of one of its helices, making the VBS accessible to vinculin. Interestingly, the talin 755-889 fragment binds more than one vinculin head molecule, suggesting that the talin rod may contain additional as yet unrecognized VBSs. |
==About this Structure== | ==About this Structure== | ||
| - | 1U6H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http:// | + | 1U6H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Barsukov, I | + | [[Category: Barsukov, I L.]] |
| - | [[Category: Critchley, D | + | [[Category: Critchley, D R.]] |
[[Category: Emsley, J.]] | [[Category: Emsley, J.]] | ||
[[Category: Fillingham, I.]] | [[Category: Fillingham, I.]] | ||
| - | [[Category: Gingras, A | + | [[Category: Gingras, A R.]] |
[[Category: Papagrigoriou, E.]] | [[Category: Papagrigoriou, E.]] | ||
[[Category: Patel, B.]] | [[Category: Patel, B.]] | ||
| - | [[Category: Roberts, G | + | [[Category: Roberts, G C.K.]] |
[[Category: protein-protein complex]] | [[Category: protein-protein complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:05 2008'' |
Revision as of 13:21, 21 February 2008
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Vinculin head (0-258) in complex with the talin vinculin binding site 2 (849-879)
Overview
The cytoskeletal protein talin plays a key role in activating integrins and in coupling them to the actin cytoskeleton. Its N-terminal globular head, which binds beta integrins, is linked to an extended rod having a C-terminal actin binding site and several vinculin binding sites (VBSs). The NMR structure of residues 755-889 of the rod (containing a VBS) is shown to be an amphipathic four-helix bundle with a left-handed topology. A talin peptide corresponding to the VBS binds the vinculin head; the X-ray crystallographic structure of this complex shows that the residues which interact with vinculin are buried in the hydrophobic core of the talin fragment. NMR shows that the interaction involves a major structural change in the talin fragment, including unfolding of one of its helices, making the VBS accessible to vinculin. Interestingly, the talin 755-889 fragment binds more than one vinculin head molecule, suggesting that the talin rod may contain additional as yet unrecognized VBSs.
About this Structure
1U6H is a Protein complex structure of sequences from Gallus gallus. Full crystallographic information is available from OCA.
Reference
A vinculin binding domain from the talin rod unfolds to form a complex with the vinculin head., Fillingham I, Gingras AR, Papagrigoriou E, Patel B, Emsley J, Critchley DR, Roberts GC, Barsukov IL, Structure. 2005 Jan;13(1):65-74. PMID:15642262
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