1u8x
From Proteopedia
(New page: 200px<br /><applet load="1u8x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u8x, resolution 2.05Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1u8x.gif|left|200px]]<br /><applet load="1u8x" size=" | + | [[Image:1u8x.gif|left|200px]]<br /><applet load="1u8x" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u8x, resolution 2.05Å" /> | caption="1u8x, resolution 2.05Å" /> | ||
'''CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE'''<br /> | '''CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the | + | GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the hydrolysis of maltose-6'-phosphate and belongs to glycoside hydrolase family GH4. GH4 enzymes are unique in their requirement for NAD(H) and a divalent metal for activity. We have determined the crystal structure of GlvA in complex with its ligands to 2.05 A resolution. Analyses of the active site architecture, in conjunction with mechanistic studies and precedent from the nucleotide diphosphate hexose dehydratases and other systems, suggest a novel mechanism of glycoside hydrolysis by GlvA that involves both the NAD(H) and the metal. |
==About this Structure== | ==About this Structure== | ||
| - | 1U8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with G6P, MN and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | + | 1U8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=G6P:'>G6P</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1NRH. Active as [http://en.wikipedia.org/wiki/Maltose-6'-phosphate_glucosidase Maltose-6'-phosphate glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.122 3.2.1.122] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Maltose-6'-phosphate glucosidase]] | [[Category: Maltose-6'-phosphate glucosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Anderson, W | + | [[Category: Anderson, W F.]] |
[[Category: Collart, F.]] | [[Category: Collart, F.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
| - | [[Category: Rajan, S | + | [[Category: Rajan, S S.]] |
[[Category: Yang, X.]] | [[Category: Yang, X.]] | ||
[[Category: G6P]] | [[Category: G6P]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:51 2008'' |
Revision as of 13:21, 21 February 2008
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CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE
Overview
GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the hydrolysis of maltose-6'-phosphate and belongs to glycoside hydrolase family GH4. GH4 enzymes are unique in their requirement for NAD(H) and a divalent metal for activity. We have determined the crystal structure of GlvA in complex with its ligands to 2.05 A resolution. Analyses of the active site architecture, in conjunction with mechanistic studies and precedent from the nucleotide diphosphate hexose dehydratases and other systems, suggest a novel mechanism of glycoside hydrolysis by GlvA that involves both the NAD(H) and the metal.
About this Structure
1U8X is a Single protein structure of sequence from Bacillus subtilis with , and as ligands. This structure supersedes the now removed PDB entry 1NRH. Active as Maltose-6'-phosphate glucosidase, with EC number 3.2.1.122 Full crystallographic information is available from OCA.
Reference
Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis., Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF, Structure. 2004 Sep;12(9):1619-29. PMID:15341727
Page seeded by OCA on Thu Feb 21 15:21:51 2008
Categories: Bacillus subtilis | Maltose-6'-phosphate glucosidase | Single protein | Anderson, W F. | Collart, F. | MCSG, Midwest Center for Structural Genomics. | Rajan, S S. | Yang, X. | G6P | MN | NAD | Glucosidase | Mcsg | Midwest center for structural genomics | Nad-dependent | Protein structure initiative | Psi | Structural genomics
