1ubd
From Proteopedia
(New page: 200px<br /> <applet load="1ubd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ubd, resolution 2.500Å" /> '''CO-CRYSTAL STRUCTU...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ubd.gif|left|200px]]<br /> | + | [[Image:1ubd.gif|left|200px]]<br /><applet load="1ubd" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ubd" size=" | + | |
caption="1ubd, resolution 2.500Å" /> | caption="1ubd, resolution 2.500Å" /> | ||
'''CO-CRYSTAL STRUCTURE OF HUMAN YY1 ZINC FINGER DOMAIN BOUND TO THE ADENO-ASSOCIATED VIRUS P5 INITIATOR ELEMENT'''<br /> | '''CO-CRYSTAL STRUCTURE OF HUMAN YY1 ZINC FINGER DOMAIN BOUND TO THE ADENO-ASSOCIATED VIRUS P5 INITIATOR ELEMENT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ying-Yang 1 protein (YY1) supports specific, unidirectional initiation of | + | Ying-Yang 1 protein (YY1) supports specific, unidirectional initiation of messenger RNA production by RNA polymerase II from two adjacent start sites in the adeno-associated virus P5 promoter, a process which is independent of the TATA box-binding protein (TBP). The 2.5-A resolution YY1-initiator element cocrystal structure reveals four zinc fingers recognizing a YY1-binding consensus sequence. Upstream of the transcription start sites protein-DNA contacts involve both strands and downstream they are virtually restricted to the template strand, permitting access to the active center of RNA polymerase II and ensuring specificity and directionality. The observed pattern of protein-DNA contacts also explains YY1 binding to a preformed transcription bubble, and YY1 binding to a DNA/RNA hybrid analog of the P5 promoter region containing a nascent RNA transcript. A model is proposed for YY1-directed, TBP-independent transcription initiation. |
==About this Structure== | ==About this Structure== | ||
| - | 1UBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1UBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UBD OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 13: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: Houbaviy, H | + | [[Category: Houbaviy, H B.]] |
[[Category: Shenk, T.]] | [[Category: Shenk, T.]] | ||
[[Category: Usheva, A.]] | [[Category: Usheva, A.]] | ||
| Line 26: | Line 25: | ||
[[Category: zinc finger protein]] | [[Category: zinc finger protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:22:37 2008'' |
Revision as of 13:22, 21 February 2008
|
CO-CRYSTAL STRUCTURE OF HUMAN YY1 ZINC FINGER DOMAIN BOUND TO THE ADENO-ASSOCIATED VIRUS P5 INITIATOR ELEMENT
Overview
Ying-Yang 1 protein (YY1) supports specific, unidirectional initiation of messenger RNA production by RNA polymerase II from two adjacent start sites in the adeno-associated virus P5 promoter, a process which is independent of the TATA box-binding protein (TBP). The 2.5-A resolution YY1-initiator element cocrystal structure reveals four zinc fingers recognizing a YY1-binding consensus sequence. Upstream of the transcription start sites protein-DNA contacts involve both strands and downstream they are virtually restricted to the template strand, permitting access to the active center of RNA polymerase II and ensuring specificity and directionality. The observed pattern of protein-DNA contacts also explains YY1 binding to a preformed transcription bubble, and YY1 binding to a DNA/RNA hybrid analog of the P5 promoter region containing a nascent RNA transcript. A model is proposed for YY1-directed, TBP-independent transcription initiation.
About this Structure
1UBD is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Cocrystal structure of YY1 bound to the adeno-associated virus P5 initiator., Houbaviy HB, Usheva A, Shenk T, Burley SK, Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13577-82. PMID:8942976
Page seeded by OCA on Thu Feb 21 15:22:37 2008
