1ucu
From Proteopedia
(New page: 200px<br /><applet load="1ucu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ucu, resolution 4.0Å" /> '''R-type straight flage...) |
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| - | [[Image:1ucu.gif|left|200px]]<br /><applet load="1ucu" size=" | + | [[Image:1ucu.gif|left|200px]]<br /><applet load="1ucu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ucu, resolution 4.0Å" /> | caption="1ucu, resolution 4.0Å" /> | ||
'''R-type straight flagellar filament made of full-length flagellin'''<br /> | '''R-type straight flagellar filament made of full-length flagellin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The bacterial flagellar filament is a helical propeller for bacterial | + | The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagellin fragment lacking about 100 terminal residues revealed the protofilament structure, but the full filament structure is still essential for understanding the mechanism of supercoiling and polymerization. Here we report a complete atomic model of the R-type filament by electron cryomicroscopy. A density map obtained from image data up to 4 A resolution shows the feature of alpha-helical backbone and some large side chains. The atomic model built on the map reveals intricate molecular packing and an alpha-helical coiled coil formed by the terminal chains in the inner core of the filament, with its intersubunit hydrophobic interactions having an important role in stabilizing the filament. |
==About this Structure== | ==About this Structure== | ||
| - | 1UCU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http:// | + | 1UCU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: helical reconstruction]] | [[Category: helical reconstruction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:10 2008'' |
Revision as of 13:23, 21 February 2008
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R-type straight flagellar filament made of full-length flagellin
Overview
The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagellin fragment lacking about 100 terminal residues revealed the protofilament structure, but the full filament structure is still essential for understanding the mechanism of supercoiling and polymerization. Here we report a complete atomic model of the R-type filament by electron cryomicroscopy. A density map obtained from image data up to 4 A resolution shows the feature of alpha-helical backbone and some large side chains. The atomic model built on the map reveals intricate molecular packing and an alpha-helical coiled coil formed by the terminal chains in the inner core of the filament, with its intersubunit hydrophobic interactions having an important role in stabilizing the filament.
About this Structure
1UCU is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy., Yonekura K, Maki-Yonekura S, Namba K, Nature. 2003 Aug 7;424(6949):643-50. PMID:12904785
Page seeded by OCA on Thu Feb 21 15:23:10 2008
