1udk
From Proteopedia
(New page: 200px<br /><applet load="1udk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1udk" /> '''Solution Structure of Nawaprin'''<br /> ==O...) |
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| - | [[Image:1udk.jpg|left|200px]]<br /><applet load="1udk" size=" | + | [[Image:1udk.jpg|left|200px]]<br /><applet load="1udk" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution Structure of Nawaprin'''<br /> | '''Solution Structure of Nawaprin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of nawaprin has been determined by nuclear | + | The three-dimensional structure of nawaprin has been determined by nuclear magnetic resonance spectroscopy. This 51-amino acid residue peptide was isolated from the venom of the spitting cobra, Naja nigricollis, and is the first member of a new family of snake venom proteins referred to as waprins. Nawaprin is relatively flat and disc-like in shape, characterized by a spiral backbone configuration that forms outer and inner circular segments. The two circular segments are held together by four disulfide bonds, three of which are clustered at the base of the molecule. The inner segment contains a short antiparallel beta-sheet, whereas the outer segment is devoid of secondary structures except for a small turn or 310 helix. The structure of nawaprin is very similar to elafin, a human leukocyte elastase-specific inhibitor. Although substantial parts of the nawaprin molecule are well defined, the tips of the outer and inner circular segments, which are hypothesized to be critical for binding interactions, are apparently disordered, similar to that found in elafin. The amino acid residues in these important regions in nawaprin are different from those in elafin, suggesting that nawaprin is not an elastase-specific inhibitor and therefore has a different function in the snake venom. |
==About this Structure== | ==About this Structure== | ||
| - | 1UDK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Naja_nigricollis Naja nigricollis]. Full crystallographic information is available from [http:// | + | 1UDK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Naja_nigricollis Naja nigricollis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UDK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Desai, M.]] | [[Category: Desai, M.]] | ||
| - | [[Category: Kini, R | + | [[Category: Kini, R M.]] |
| - | [[Category: Kuchel, P | + | [[Category: Kuchel, P W.]] |
[[Category: Moochhala, S.]] | [[Category: Moochhala, S.]] | ||
| - | [[Category: Torres, A | + | [[Category: Torres, A M.]] |
| - | [[Category: Wong, H | + | [[Category: Wong, H Y.]] |
[[Category: antiparallel beta-sheet]] | [[Category: antiparallel beta-sheet]] | ||
[[Category: spiral backbone configuration]] | [[Category: spiral backbone configuration]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:20 2008'' |
Revision as of 13:23, 21 February 2008
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Solution Structure of Nawaprin
Overview
The three-dimensional structure of nawaprin has been determined by nuclear magnetic resonance spectroscopy. This 51-amino acid residue peptide was isolated from the venom of the spitting cobra, Naja nigricollis, and is the first member of a new family of snake venom proteins referred to as waprins. Nawaprin is relatively flat and disc-like in shape, characterized by a spiral backbone configuration that forms outer and inner circular segments. The two circular segments are held together by four disulfide bonds, three of which are clustered at the base of the molecule. The inner segment contains a short antiparallel beta-sheet, whereas the outer segment is devoid of secondary structures except for a small turn or 310 helix. The structure of nawaprin is very similar to elafin, a human leukocyte elastase-specific inhibitor. Although substantial parts of the nawaprin molecule are well defined, the tips of the outer and inner circular segments, which are hypothesized to be critical for binding interactions, are apparently disordered, similar to that found in elafin. The amino acid residues in these important regions in nawaprin are different from those in elafin, suggesting that nawaprin is not an elastase-specific inhibitor and therefore has a different function in the snake venom.
About this Structure
1UDK is a Protein complex structure of sequences from Naja nigricollis. Full crystallographic information is available from OCA.
Reference
Identification of a novel family of proteins in snake venoms. Purification and structural characterization of nawaprin from Naja nigricollis snake venom., Torres AM, Wong HY, Desai M, Moochhala S, Kuchel PW, Kini RM, J Biol Chem. 2003 Oct 10;278(41):40097-104. Epub 2003 Jul 23. PMID:12878611
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