1udv
From Proteopedia
(New page: 200px<br /><applet load="1udv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1udv, resolution 1.85Å" /> '''Crystal structure of...) |
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| - | [[Image:1udv.jpg|left|200px]]<br /><applet load="1udv" size=" | + | [[Image:1udv.jpg|left|200px]]<br /><applet load="1udv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1udv, resolution 1.85Å" /> | caption="1udv, resolution 1.85Å" /> | ||
'''Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A'''<br /> | '''Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a small, basic DNA binding protein, Sso10b2, from | + | The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure is similar to that of Sso10b1 (also called Alba) from the same organism. However, Sso10b2 contains an arginine-rich loop RDRRR motif, which may play an important role in nucleic acid binding. There are two independent Sso10b2 proteins in the asymmetric unit, and a plausible stable dimer could be deduced from the crystal structure. Topology comparison revealed that Sso10b2 is similar to several RNA-binding proteins, including IF3-C, YhhP, and DNase I. Models of the Sso10b2 dimer bound to either B-DNA or A-DNA have been constructed. |
==About this Structure== | ==About this Structure== | ||
| - | 1UDV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1UDV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UDV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
| - | [[Category: Chen, C | + | [[Category: Chen, C Y.]] |
| - | [[Category: Chou, C | + | [[Category: Chou, C C.]] |
| - | [[Category: Lin, T | + | [[Category: Lin, T W.]] |
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: dna binding protein]] | [[Category: dna binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:26 2008'' |
Revision as of 13:23, 21 February 2008
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Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A
Overview
The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure is similar to that of Sso10b1 (also called Alba) from the same organism. However, Sso10b2 contains an arginine-rich loop RDRRR motif, which may play an important role in nucleic acid binding. There are two independent Sso10b2 proteins in the asymmetric unit, and a plausible stable dimer could be deduced from the crystal structure. Topology comparison revealed that Sso10b2 is similar to several RNA-binding proteins, including IF3-C, YhhP, and DNase I. Models of the Sso10b2 dimer bound to either B-DNA or A-DNA have been constructed.
About this Structure
1UDV is a Single protein structure of sequence from Sulfolobus solfataricus with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms., Chou CC, Lin TW, Chen CY, Wang AH, J Bacteriol. 2003 Jul;185(14):4066-73. PMID:12837780
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