1udw
From Proteopedia
(New page: 200px<br /> <applet load="1udw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1udw, resolution 2.60Å" /> '''Crystal structure o...) |
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| - | [[Image:1udw.gif|left|200px]]<br /> | + | [[Image:1udw.gif|left|200px]]<br /><applet load="1udw" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1udw, resolution 2.60Å" /> | caption="1udw, resolution 2.60Å" /> | ||
'''Crystal structure of human uridine-cytidine kinase 2 complexed with a feedback-inhibitor, CTP'''<br /> | '''Crystal structure of human uridine-cytidine kinase 2 complexed with a feedback-inhibitor, CTP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and | + | Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and cytidine and activates pharmacological ribonucleoside analogs. Here we present the crystal structures of human UCK alone and in complexes with a substrate, cytidine, a feedback inhibitor, CTP or UTP, and with phosphorylation products, CMP and ADP, respectively. Free UCK takes an alpha/beta mononucleotide binding fold and exists as a homotetramer with 222 symmetry. Upon inhibitor binding, one loop region was loosened, causing the UCK tetramer to be distorted. Upon cytidine binding, a large induced fit was observed at the uridine/cytidine binding site, which endows UCK with a strict specificity for pyrimidine ribonucleosides. The first UCK structure provided the structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase, which give clues for the design of novel antitumor and antiviral ribonucleoside analogs that inhibit RNA synthesis. |
==About this Structure== | ==About this Structure== | ||
| - | 1UDW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CTP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Uridine_kinase Uridine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.48 2.7.1.48] Full crystallographic information is available from [http:// | + | 1UDW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CTP:'>CTP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Uridine_kinase Uridine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.48 2.7.1.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UDW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Koizumi, K.]] | [[Category: Koizumi, K.]] | ||
[[Category: Matsuda, A.]] | [[Category: Matsuda, A.]] | ||
| - | [[Category: Suzuki, N | + | [[Category: Suzuki, N N.]] |
[[Category: CTP]] | [[Category: CTP]] | ||
[[Category: alpha/beta mononucleotide-binding hold]] | [[Category: alpha/beta mononucleotide-binding hold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:24 2008'' |
Revision as of 13:23, 21 February 2008
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Crystal structure of human uridine-cytidine kinase 2 complexed with a feedback-inhibitor, CTP
Overview
Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and cytidine and activates pharmacological ribonucleoside analogs. Here we present the crystal structures of human UCK alone and in complexes with a substrate, cytidine, a feedback inhibitor, CTP or UTP, and with phosphorylation products, CMP and ADP, respectively. Free UCK takes an alpha/beta mononucleotide binding fold and exists as a homotetramer with 222 symmetry. Upon inhibitor binding, one loop region was loosened, causing the UCK tetramer to be distorted. Upon cytidine binding, a large induced fit was observed at the uridine/cytidine binding site, which endows UCK with a strict specificity for pyrimidine ribonucleosides. The first UCK structure provided the structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase, which give clues for the design of novel antitumor and antiviral ribonucleoside analogs that inhibit RNA synthesis.
About this Structure
1UDW is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Uridine kinase, with EC number 2.7.1.48 Full crystallographic information is available from OCA.
Reference
Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase., Suzuki NN, Koizumi K, Fukushima M, Matsuda A, Inagaki F, Structure. 2004 May;12(5):751-64. PMID:15130468
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