1ue5
From Proteopedia
(New page: 200px<br /><applet load="1ue5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ue5, resolution 2.60Å" /> '''Crystal structure of...) |
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| - | [[Image:1ue5.gif|left|200px]]<br /><applet load="1ue5" size=" | + | [[Image:1ue5.gif|left|200px]]<br /><applet load="1ue5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ue5, resolution 2.60Å" /> | caption="1ue5, resolution 2.60Å" /> | ||
'''Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis'''<br /> | '''Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Single-stranded DNA-binding protein (SSB) is an essential protein | + | Single-stranded DNA-binding protein (SSB) is an essential protein necessary for the functioning of the DNA replication, repair and recombination machineries. Here we report the structure of the DNA-binding domain of Mycobacterium tuberculosis SSB (MtuSSB) in four different crystals distributed in two forms. The structure of one of the forms was solved by a combination of isomorphous replacement and anomalous scattering. This structure was used to determine the structure of the other form by molecular replacement. The polypeptide chain in the structure exhibits the oligonucleotide binding fold. The globular core of the molecule in different subunits in the two forms and those in Escherichia coli SSB (EcoSSB) and human mitochondrial SSB (HMtSSB) have similar structure, although the three loops exhibit considerable structural variation. However, the tetrameric MtuSSB has an as yet unobserved quaternary association. This quaternary structure with a unique dimeric interface lends the oligomeric protein greater stability, which may be of significance to the functioning of the protein under conditions of stress. Also, as a result of the variation in the quaternary structure the path adopted by the DNA to wrap around MtuSSB is expected to be different from that of EcoSSB. |
==About this Structure== | ==About this Structure== | ||
| - | 1UE5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1UE5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saikrishnan, K.]] | [[Category: Saikrishnan, K.]] | ||
[[Category: Sekar, K.]] | [[Category: Sekar, K.]] | ||
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
[[Category: Varshney, U.]] | [[Category: Varshney, U.]] | ||
[[Category: Venkatesh, J.]] | [[Category: Venkatesh, J.]] | ||
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[[Category: tbsgc]] | [[Category: tbsgc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:34 2008'' |
Revision as of 13:23, 21 February 2008
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Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis
Overview
Single-stranded DNA-binding protein (SSB) is an essential protein necessary for the functioning of the DNA replication, repair and recombination machineries. Here we report the structure of the DNA-binding domain of Mycobacterium tuberculosis SSB (MtuSSB) in four different crystals distributed in two forms. The structure of one of the forms was solved by a combination of isomorphous replacement and anomalous scattering. This structure was used to determine the structure of the other form by molecular replacement. The polypeptide chain in the structure exhibits the oligonucleotide binding fold. The globular core of the molecule in different subunits in the two forms and those in Escherichia coli SSB (EcoSSB) and human mitochondrial SSB (HMtSSB) have similar structure, although the three loops exhibit considerable structural variation. However, the tetrameric MtuSSB has an as yet unobserved quaternary association. This quaternary structure with a unique dimeric interface lends the oligomeric protein greater stability, which may be of significance to the functioning of the protein under conditions of stress. Also, as a result of the variation in the quaternary structure the path adopted by the DNA to wrap around MtuSSB is expected to be different from that of EcoSSB.
About this Structure
1UE5 is a Single protein structure of sequence from Mycobacterium tuberculosis with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M, J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:12888346
Page seeded by OCA on Thu Feb 21 15:23:34 2008
Categories: Mycobacterium tuberculosis | Single protein | Acharya, N. | Jeyakanthan, J. | Saikrishnan, K. | Sekar, K. | TBSGC, TB Structural Genomics Consortium. | Varshney, U. | Venkatesh, J. | Vijayan, M. | CD | Dna-binding protein | Oligonucleotide binding fold | Protein structure initiative | Psi | Structural genomics | Tb structural genomics consortium | Tbsgc
