1gn9

From Proteopedia

Revision as of 13:10, 30 October 2007

HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 X-RAY STRUCTURE AT 2.6A RESOLUTION USING SYNCHROTRON RADIATION AT A WAVELENGTH OF 1.722A

Overview

The structures of the hybrid cluster proteins (HCPs) from the, sulfate-reducing bacteria Desulfovibrio desulfuricans (ATCC 27774) and, Desulfovibrio vulgaris (Hildenborough) have been elucidated at a, resolution of 1.25 A using X-ray synchrotron radiation techniques. In the, case of the D. desulfuricans protein, protein isolation, purification, crystallization and X-ray data collection were carried out under strict, anaerobic conditions, whereas for the D. vulgaris protein the conditions, were aerobic. However, both structures are essentially the same, comprising three domains and two iron-sulfur centres. One of these centres, situated near the exterior of the molecules in domain 1 is a cubane, [4Fe-4S] cluster, whereas the other, located at the interface of the three, domains, contains ... [(full description)]

About this Structure

1GN9 is a [Single protein] structure of sequence from [Desulfovibrio desulfuricans] with FSO and SF4 as [ligands]. Structure known Active Site: CSA. Full crystallographic information is available from [OCA].

Reference

Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 A resolution using synchrotron radiation., Macedo S, Mitchell EP, Romao CV, Cooper SJ, Coelho R, Liu MY, Xavier AV, LeGall J, Bailey S, Garner DC, Hagen WR, Teixeira M, Carrondo MA, Lindley P, J Biol Inorg Chem. 2002 Apr;7(4-5):514-25. Epub 2002 Jan 23. PMID:11941509

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