1ugl
From Proteopedia
(New page: 200px<br /><applet load="1ugl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ugl" /> '''Solution structure of S8-SP11'''<br /> ==Ov...) |
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| - | [[Image:1ugl.jpg|left|200px]]<br /><applet load="1ugl" size=" | + | [[Image:1ugl.jpg|left|200px]]<br /><applet load="1ugl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ugl" /> | caption="1ugl" /> | ||
'''Solution structure of S8-SP11'''<br /> | '''Solution structure of S8-SP11'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Many flowering plants possess a self-incompatibility system to prevent | + | Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor. |
==About this Structure== | ==About this Structure== | ||
| - | 1UGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1UGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UGL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Isogai, A.]] | [[Category: Isogai, A.]] | ||
| - | [[Category: Jee, J | + | [[Category: Jee, J G.]] |
[[Category: Kojima, C.]] | [[Category: Kojima, C.]] | ||
[[Category: Mishima, M.]] | [[Category: Mishima, M.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Sasaki, K.]] | [[Category: Sasaki, K.]] | ||
[[Category: Shirakawa, M.]] | [[Category: Shirakawa, M.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:24:15 2008'' |
Revision as of 13:24, 21 February 2008
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Solution structure of S8-SP11
Overview
Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor.
About this Structure
1UGL is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the male determinant factor for Brassica self-incompatibility., Mishima M, Takayama S, Sasaki K, Jee JG, Kojima C, Isogai A, Shirakawa M, J Biol Chem. 2003 Sep 19;278(38):36389-95. Epub 2003 Jun 30. PMID:12835321
Page seeded by OCA on Thu Feb 21 15:24:15 2008
Categories: Single protein | Isogai, A. | Jee, J G. | Kojima, C. | Mishima, M. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Sasaki, K. | Shirakawa, M. | Takayama, S. | Cysteine-rich | Defensin-like | Male determinant of self-incompatibility | Nmr | Riken structural genomics/proteomics initiative | Rsgi | Scr | Sp11 | Structural genomics
