1uj2
From Proteopedia
(New page: 200px<br /> <applet load="1uj2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uj2, resolution 1.80Å" /> '''Crystal structure o...) |
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| - | [[Image:1uj2.gif|left|200px]]<br /> | + | [[Image:1uj2.gif|left|200px]]<br /><applet load="1uj2" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1uj2" size=" | + | |
caption="1uj2, resolution 1.80Å" /> | caption="1uj2, resolution 1.80Å" /> | ||
'''Crystal structure of human uridine-cytidine kinase 2 complexed with products, CMP and ADP'''<br /> | '''Crystal structure of human uridine-cytidine kinase 2 complexed with products, CMP and ADP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and | + | Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and cytidine and activates pharmacological ribonucleoside analogs. Here we present the crystal structures of human UCK alone and in complexes with a substrate, cytidine, a feedback inhibitor, CTP or UTP, and with phosphorylation products, CMP and ADP, respectively. Free UCK takes an alpha/beta mononucleotide binding fold and exists as a homotetramer with 222 symmetry. Upon inhibitor binding, one loop region was loosened, causing the UCK tetramer to be distorted. Upon cytidine binding, a large induced fit was observed at the uridine/cytidine binding site, which endows UCK with a strict specificity for pyrimidine ribonucleosides. The first UCK structure provided the structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase, which give clues for the design of novel antitumor and antiviral ribonucleoside analogs that inhibit RNA synthesis. |
==About this Structure== | ==About this Structure== | ||
| - | 1UJ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, C5P and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Uridine_kinase Uridine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.48 2.7.1.48] Full crystallographic information is available from [http:// | + | 1UJ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=C5P:'>C5P</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Uridine_kinase Uridine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.48 2.7.1.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJ2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Koizumi, K.]] | [[Category: Koizumi, K.]] | ||
[[Category: Matsuda, A.]] | [[Category: Matsuda, A.]] | ||
| - | [[Category: Suzuki, N | + | [[Category: Suzuki, N N.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: C5P]] | [[Category: C5P]] | ||
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[[Category: alpha/beta mononucleotide-binding hold]] | [[Category: alpha/beta mononucleotide-binding hold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:04 2008'' |
Revision as of 13:25, 21 February 2008
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Crystal structure of human uridine-cytidine kinase 2 complexed with products, CMP and ADP
Overview
Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and cytidine and activates pharmacological ribonucleoside analogs. Here we present the crystal structures of human UCK alone and in complexes with a substrate, cytidine, a feedback inhibitor, CTP or UTP, and with phosphorylation products, CMP and ADP, respectively. Free UCK takes an alpha/beta mononucleotide binding fold and exists as a homotetramer with 222 symmetry. Upon inhibitor binding, one loop region was loosened, causing the UCK tetramer to be distorted. Upon cytidine binding, a large induced fit was observed at the uridine/cytidine binding site, which endows UCK with a strict specificity for pyrimidine ribonucleosides. The first UCK structure provided the structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase, which give clues for the design of novel antitumor and antiviral ribonucleoside analogs that inhibit RNA synthesis.
About this Structure
1UJ2 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as Uridine kinase, with EC number 2.7.1.48 Full crystallographic information is available from OCA.
Reference
Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase., Suzuki NN, Koizumi K, Fukushima M, Matsuda A, Inagaki F, Structure. 2004 May;12(5):751-64. PMID:15130468
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