1uis
From Proteopedia
(New page: 200px<br /><applet load="1uis" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uis, resolution 2.0Å" /> '''The 2.0 crystal struc...) |
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| - | [[Image:1uis.gif|left|200px]]<br /><applet load="1uis" size=" | + | [[Image:1uis.gif|left|200px]]<br /><applet load="1uis" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uis, resolution 2.0Å" /> | caption="1uis, resolution 2.0Å" /> | ||
'''The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor'''<br /> | '''The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have crystallized and subsequently determined to 2.0-A resolution the | + | We have crystallized and subsequently determined to 2.0-A resolution the crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor. The structure of the protomer, which adopts a beta-can topology, is similar to that of the related monomeric green fluorescent protein (GFP). The quaternary structure of eqFP611, a tetramer exhibiting 222 symmetry, is similar to that observed for the more closely related red fluorescent protein DsRed and the chromoprotein Rtms5. The unique chromophore sequence (Met63-Tyr64-Gly65) of eqFP611, adopts a coplanar and trans conformation within the interior of the beta-can fold. Accordingly, the eqFP611 chromophore adopts a significantly different conformation in comparison to the chromophore conformation observed in GFP, DsRed, and Rtms5. The coplanar chromophore conformation and its immediate environment provide a structural basis for the far red, highly fluorescent nature of eqFP611. The eqFP611 structure extends our knowledge on the range of conformations a chromophore can adopt within closely related members of the green fluorescent protein family. |
==About this Structure== | ==About this Structure== | ||
| - | 1UIS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor] with CA and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1UIS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UIS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Beddoe, T.]] | [[Category: Beddoe, T.]] | ||
| - | [[Category: Devenish, R | + | [[Category: Devenish, R J.]] |
| - | [[Category: Oakley, A | + | [[Category: Oakley, A J.]] |
[[Category: Petersen, J.]] | [[Category: Petersen, J.]] | ||
[[Category: Prescott, M.]] | [[Category: Prescott, M.]] | ||
[[Category: Rossjohn, J.]] | [[Category: Rossjohn, J.]] | ||
| - | [[Category: Wilmann, P | + | [[Category: Wilmann, P G.]] |
[[Category: ACY]] | [[Category: ACY]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: luminescent protein]] | [[Category: luminescent protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:03 2008'' |
Revision as of 13:25, 21 February 2008
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The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor
Overview
We have crystallized and subsequently determined to 2.0-A resolution the crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor. The structure of the protomer, which adopts a beta-can topology, is similar to that of the related monomeric green fluorescent protein (GFP). The quaternary structure of eqFP611, a tetramer exhibiting 222 symmetry, is similar to that observed for the more closely related red fluorescent protein DsRed and the chromoprotein Rtms5. The unique chromophore sequence (Met63-Tyr64-Gly65) of eqFP611, adopts a coplanar and trans conformation within the interior of the beta-can fold. Accordingly, the eqFP611 chromophore adopts a significantly different conformation in comparison to the chromophore conformation observed in GFP, DsRed, and Rtms5. The coplanar chromophore conformation and its immediate environment provide a structural basis for the far red, highly fluorescent nature of eqFP611. The eqFP611 structure extends our knowledge on the range of conformations a chromophore can adopt within closely related members of the green fluorescent protein family.
About this Structure
1UIS is a Single protein structure of sequence from Entacmaea quadricolor with and as ligands. Full crystallographic information is available from OCA.
Reference
The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor., Petersen J, Wilmann PG, Beddoe T, Oakley AJ, Devenish RJ, Prescott M, Rossjohn J, J Biol Chem. 2003 Nov 7;278(45):44626-31. Epub 2003 Aug 8. PMID:12909624
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