1ujw
From Proteopedia
(New page: 200px<br /><applet load="1ujw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ujw, resolution 2.75Å" /> '''Structure of the com...) |
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| - | [[Image:1ujw.gif|left|200px]]<br /><applet load="1ujw" size=" | + | [[Image:1ujw.gif|left|200px]]<br /><applet load="1ujw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ujw, resolution 2.75Å" /> | caption="1ujw, resolution 2.75Å" /> | ||
'''Structure of the complex between BtuB and Colicin E3 Receptor binding domain'''<br /> | '''Structure of the complex between BtuB and Colicin E3 Receptor binding domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cellular import of colicin E3 is initiated by the Escherichia coli outer | + | Cellular import of colicin E3 is initiated by the Escherichia coli outer membrane cobalamin transporter, BtuB. The 135-residue 100-A coiled-coil receptor-binding domain (R135) of colicin E3 forms a 1:1 complex with BtuB whose structure at a resolution of 2.75 A is reported. Binding of R135 to the BtuB extracellular surface (DeltaG(o) = -12 kcal mol(-1)) is mediated by 27 residues of R135 near the coiled-coil apex. Formation of the R135-BtuB complex results in unfolding of R135 N- and C-terminal ends, inferred to be important for unfolding of the colicin T-domain. Small conformational changes occur in the BtuB cork and barrel domains but are insufficient to form a translocation channel. The absence of a channel and the peripheral binding of R135 imply that BtuB serves to bind the colicin, and that the coiled-coil delivers the colicin to a neighboring outer membrane protein for translocation, thus forming a colicin translocon. The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3. |
==About this Structure== | ==About this Structure== | ||
| - | 1UJW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GP1, LDA, AAE, LIM and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1UJW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GP1:'>GP1</scene>, <scene name='pdbligand=LDA:'>LDA</scene>, <scene name='pdbligand=AAE:'>AAE</scene>, <scene name='pdbligand=LIM:'>LIM</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Antonenko, Y | + | [[Category: Antonenko, Y N.]] |
[[Category: Bano, S.]] | [[Category: Bano, S.]] | ||
| - | [[Category: Cramer, W | + | [[Category: Cramer, W A.]] |
| - | [[Category: Eroukova, V | + | [[Category: Eroukova, V Y.]] |
[[Category: Kurisu, G.]] | [[Category: Kurisu, G.]] | ||
| - | [[Category: Rokitskaya, T | + | [[Category: Rokitskaya, T I.]] |
| - | [[Category: Wiener, M | + | [[Category: Wiener, M C.]] |
| - | [[Category: Zakharov, S | + | [[Category: Zakharov, S D.]] |
| - | [[Category: Zhalnina, M | + | [[Category: Zhalnina, M V.]] |
[[Category: AAE]] | [[Category: AAE]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:16 2008'' |
Revision as of 13:25, 21 February 2008
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Structure of the complex between BtuB and Colicin E3 Receptor binding domain
Overview
Cellular import of colicin E3 is initiated by the Escherichia coli outer membrane cobalamin transporter, BtuB. The 135-residue 100-A coiled-coil receptor-binding domain (R135) of colicin E3 forms a 1:1 complex with BtuB whose structure at a resolution of 2.75 A is reported. Binding of R135 to the BtuB extracellular surface (DeltaG(o) = -12 kcal mol(-1)) is mediated by 27 residues of R135 near the coiled-coil apex. Formation of the R135-BtuB complex results in unfolding of R135 N- and C-terminal ends, inferred to be important for unfolding of the colicin T-domain. Small conformational changes occur in the BtuB cork and barrel domains but are insufficient to form a translocation channel. The absence of a channel and the peripheral binding of R135 imply that BtuB serves to bind the colicin, and that the coiled-coil delivers the colicin to a neighboring outer membrane protein for translocation, thus forming a colicin translocon. The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3.
About this Structure
1UJW is a Protein complex structure of sequences from Escherichia coli with , , , and as ligands. Full crystallographic information is available from OCA.
Reference
The structure of BtuB with bound colicin E3 R-domain implies a translocon., Kurisu G, Zakharov SD, Zhalnina MV, Bano S, Eroukova VY, Rokitskaya TI, Antonenko YN, Wiener MC, Cramer WA, Nat Struct Biol. 2003 Nov;10(11):948-54. Epub 2003 Oct 5. PMID:14528295
Page seeded by OCA on Thu Feb 21 15:25:16 2008
Categories: Escherichia coli | Protein complex | Antonenko, Y N. | Bano, S. | Cramer, W A. | Eroukova, V Y. | Kurisu, G. | Rokitskaya, T I. | Wiener, M C. | Zakharov, S D. | Zhalnina, M V. | AAE | GOL | GP1 | LDA | LIM | Beta-barrel | Coiled-coil
