1ukv
From Proteopedia
(New page: 200px<br /><applet load="1ukv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ukv, resolution 1.50Å" /> '''Structure of RabGDP-...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ukv.gif|left|200px]]<br /><applet load="1ukv" size=" | + | [[Image:1ukv.gif|left|200px]]<br /><applet load="1ukv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ukv, resolution 1.50Å" /> | caption="1ukv, resolution 1.50Å" /> | ||
'''Structure of RabGDP-dissociation inhibitor in complex with prenylated YPT1 GTPase'''<br /> | '''Structure of RabGDP-dissociation inhibitor in complex with prenylated YPT1 GTPase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rab/Ypt guanosine triphosphatases (GTPases) represent a family of key | + | Rab/Ypt guanosine triphosphatases (GTPases) represent a family of key membrane traffic regulators in eukaryotic cells whose function is governed by the guanosine diphosphate (GDP) dissociation inhibitor (RabGDI). Using a combination of chemical synthesis and protein engineering, we generated and crystallized the monoprenylated Ypt1:RabGDI complex. The structure of the complex was solved to 1.5 angstrom resolution and provides a structural basis for the ability of RabGDI to inhibit the release of nucleotide by Rab proteins. Isoprenoid binding requires a conformational change that opens a cavity in the hydrophobic core of its domain II. Analysis of the structure provides a molecular basis for understanding a RabGDI mutant that causes mental retardation in humans. |
==About this Structure== | ==About this Structure== | ||
| - | 1UKV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG, GER and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1UKV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GER:'>GER</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UKV OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: Brunsveld, L.]] | [[Category: Brunsveld, L.]] | ||
[[Category: Durek, T.]] | [[Category: Durek, T.]] | ||
| - | [[Category: Goody, R | + | [[Category: Goody, R S.]] |
[[Category: Kushnir, S.]] | [[Category: Kushnir, S.]] | ||
[[Category: Pylypenko, O.]] | [[Category: Pylypenko, O.]] | ||
| Line 30: | Line 30: | ||
[[Category: vesicular transport]] | [[Category: vesicular transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:37 2008'' |
Revision as of 13:25, 21 February 2008
|
Structure of RabGDP-dissociation inhibitor in complex with prenylated YPT1 GTPase
Overview
Rab/Ypt guanosine triphosphatases (GTPases) represent a family of key membrane traffic regulators in eukaryotic cells whose function is governed by the guanosine diphosphate (GDP) dissociation inhibitor (RabGDI). Using a combination of chemical synthesis and protein engineering, we generated and crystallized the monoprenylated Ypt1:RabGDI complex. The structure of the complex was solved to 1.5 angstrom resolution and provides a structural basis for the ability of RabGDI to inhibit the release of nucleotide by Rab proteins. Isoprenoid binding requires a conformational change that opens a cavity in the hydrophobic core of its domain II. Analysis of the structure provides a molecular basis for understanding a RabGDI mutant that causes mental retardation in humans.
About this Structure
1UKV is a Protein complex structure of sequences from Saccharomyces cerevisiae with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase., Rak A, Pylypenko O, Durek T, Watzke A, Kushnir S, Brunsveld L, Waldmann H, Goody RS, Alexandrov K, Science. 2003 Oct 24;302(5645):646-50. PMID:14576435
Page seeded by OCA on Thu Feb 21 15:25:37 2008
