1umh
From Proteopedia
(New page: 200px<br /><applet load="1umh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1umh, resolution 2.00Å" /> '''Structural basis of ...) |
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| - | [[Image:1umh.gif|left|200px]]<br /><applet load="1umh" size=" | + | [[Image:1umh.gif|left|200px]]<br /><applet load="1umh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1umh, resolution 2.00Å" /> | caption="1umh, resolution 2.00Å" /> | ||
'''Structural basis of sugar-recognizing ubiquitin ligase'''<br /> | '''Structural basis of sugar-recognizing ubiquitin ligase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | SCF(Fbs1) is a ubiquitin ligase that functions in the endoplasmic | + | SCF(Fbs1) is a ubiquitin ligase that functions in the endoplasmic reticulum (ER)-associated degradation pathway. Fbs1/Fbx2, a member of the F-box proteins, recognizes high-mannose oligosaccharides. Efficient binding to an N-glycan requires di-N-acetylchitobiose (chitobiose). Here we report the crystal structures of the sugar-binding domain (SBD) of Fbs1 alone and in complex with chitobiose. The SBD is composed of a ten-stranded antiparallel beta-sandwich. The structure of the SBD-chitobiose complex includes hydrogen bonds between Fbs1 and chitobiose and insertion of the methyl group of chitobiose into a small hydrophobic pocket of Fbs1. Moreover, NMR spectroscopy has demonstrated that the amino acid residues adjoining the chitobiose-binding site interact with the outer branches of the carbohydrate moiety. Considering that the innermost chitobiose moieties in N-glycans are usually involved in intramolecular interactions with the polypeptide moieties, we propose that Fbs1 interacts with the chitobiose in unfolded N-glycoprotein, pointing the protein moiety toward E2 for ubiquitination. |
==About this Structure== | ==About this Structure== | ||
| - | 1UMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http:// | + | 1UMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Iwai, K.]] | [[Category: Iwai, K.]] | ||
[[Category: Kato, K.]] | [[Category: Kato, K.]] | ||
| - | [[Category: Lee, S | + | [[Category: Lee, S J.]] |
[[Category: Mizushima, T.]] | [[Category: Mizushima, T.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Tanaka, K.]] | [[Category: Tanaka, K.]] | ||
[[Category: Tsukihara, T.]] | [[Category: Tsukihara, T.]] | ||
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[[Category: ubiquitin ligase]] | [[Category: ubiquitin ligase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:26:05 2008'' |
Revision as of 13:26, 21 February 2008
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Structural basis of sugar-recognizing ubiquitin ligase
Overview
SCF(Fbs1) is a ubiquitin ligase that functions in the endoplasmic reticulum (ER)-associated degradation pathway. Fbs1/Fbx2, a member of the F-box proteins, recognizes high-mannose oligosaccharides. Efficient binding to an N-glycan requires di-N-acetylchitobiose (chitobiose). Here we report the crystal structures of the sugar-binding domain (SBD) of Fbs1 alone and in complex with chitobiose. The SBD is composed of a ten-stranded antiparallel beta-sandwich. The structure of the SBD-chitobiose complex includes hydrogen bonds between Fbs1 and chitobiose and insertion of the methyl group of chitobiose into a small hydrophobic pocket of Fbs1. Moreover, NMR spectroscopy has demonstrated that the amino acid residues adjoining the chitobiose-binding site interact with the outer branches of the carbohydrate moiety. Considering that the innermost chitobiose moieties in N-glycans are usually involved in intramolecular interactions with the polypeptide moieties, we propose that Fbs1 interacts with the chitobiose in unfolded N-glycoprotein, pointing the protein moiety toward E2 for ubiquitination.
About this Structure
1UMH is a Single protein structure of sequence from Mus musculus with as ligand. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Full crystallographic information is available from OCA.
Reference
Structural basis of sugar-recognizing ubiquitin ligase., Mizushima T, Hirao T, Yoshida Y, Lee SJ, Chiba T, Iwai K, Yamaguchi Y, Kato K, Tsukihara T, Tanaka K, Nat Struct Mol Biol. 2004 Apr;11(4):365-70. Epub 2004 Feb 29. PMID:14990996
Page seeded by OCA on Thu Feb 21 15:26:05 2008
Categories: Mus musculus | Single protein | Ubiquitin--protein ligase | Chiba, T. | Hirao, T. | Iwai, K. | Kato, K. | Lee, S J. | Mizushima, T. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Tanaka, K. | Tsukihara, T. | Yamaguchi, Y. | Yoshida, Y. | NI | Lectin | Riken structural genomics/proteomics initiative | Rsgi | Scf | Structural genomics | Ubiquitin | Ubiquitin ligase
