1unk
From Proteopedia
(New page: 200px<br /><applet load="1unk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1unk, resolution 1.8Å" /> '''STRUCTURE OF COLICIN ...) |
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| - | [[Image:1unk.gif|left|200px]]<br /><applet load="1unk" size=" | + | [[Image:1unk.gif|left|200px]]<br /><applet load="1unk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1unk, resolution 1.8Å" /> | caption="1unk, resolution 1.8Å" /> | ||
'''STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN'''<br /> | '''STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Site-specific cleavage of mRNA has been identified in vivo for the | + | Site-specific cleavage of mRNA has been identified in vivo for the polycistronic colicin E7 operon (ColE7), which occurs between G and A nucleotides located at the Asp52 codon (GAT) of the immunity gene (ceiE7). In vitro, this specific cleavage occurs only in the presence of the ceiE7 gene product (ImmE7). The crystal structure of dimeric ImmE7 has been determined at 1.8 A resolution by X-ray crystallographic analysis. We found that several residues located at the interface of dimeric ImmE7 bear surprising resemblance to the active sites of some RNases. These results suggest that dimeric ImmE7 may possess a novel RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream celE7 gene as well as degradation of the upstream ceaE7 mRNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1UNK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1UNK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UNK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chak, K | + | [[Category: Chak, K F.]] |
| - | [[Category: Hsieh, S | + | [[Category: Hsieh, S Y.]] |
| - | [[Category: Ko, T | + | [[Category: Ko, T P.]] |
| - | [[Category: Ku, W | + | [[Category: Ku, W Y.]] |
| - | [[Category: Tseng, M | + | [[Category: Tseng, M Y.]] |
| - | [[Category: Yuan, H | + | [[Category: Yuan, H S.]] |
[[Category: dimeric structure]] | [[Category: dimeric structure]] | ||
[[Category: immunity protein]] | [[Category: immunity protein]] | ||
[[Category: rnase active site]] | [[Category: rnase active site]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:26:26 2008'' |
Revision as of 13:26, 21 February 2008
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STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN
Overview
Site-specific cleavage of mRNA has been identified in vivo for the polycistronic colicin E7 operon (ColE7), which occurs between G and A nucleotides located at the Asp52 codon (GAT) of the immunity gene (ceiE7). In vitro, this specific cleavage occurs only in the presence of the ceiE7 gene product (ImmE7). The crystal structure of dimeric ImmE7 has been determined at 1.8 A resolution by X-ray crystallographic analysis. We found that several residues located at the interface of dimeric ImmE7 bear surprising resemblance to the active sites of some RNases. These results suggest that dimeric ImmE7 may possess a novel RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream celE7 gene as well as degradation of the upstream ceaE7 mRNA.
About this Structure
1UNK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A novel role of ImmE7 in the autoregulatory expression of the ColE7 operon and identification of possible RNase active sites in the crystal structure of dimeric ImmE7., Hsieh SY, Ko TP, Tseng MY, Ku W, Chak KF, Yuan HS, EMBO J. 1997 Mar 17;16(6):1444-54. PMID:9135159
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