1uns
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The previously determined crystal structure of the superantigen | + | The previously determined crystal structure of the superantigen staphylococcal enterotoxin C2 (SEC2) showed binding of a single zinc ion located between the N- and C-terminal domains. Here we present the crystal structure of SEC2 determined to 2.0 A resolution in the presence of additional zinc. The structure revealed the presence of a secondary zinc-binding site close to the major histocompatibility complex (MHC)-binding site of the toxin and some 28 A away from the primary zinc-binding site of the toxin found in previous studies. T cell stimulation assays showed that varying the concentration of zinc ions present affected the activity of the toxin and we observed that high zinc concentrations considerably inhibited T cell responses. This indicates that SEC2 may have multiple modes of interaction with the immune system that are dependent on serum zinc levels. The potential role of the secondary zinc-binding site and that of the primary one in the formation of the TCR.SEC2.MHC complex are considered, and the possibility that zinc may regulate the activity of SEC2 as a toxin facilitating different T cell responses is discussed. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
| - | [[Category: Acharya, K | + | [[Category: Acharya, K R.]] |
| - | [[Category: Baker, M | + | [[Category: Baker, M D.]] |
[[Category: Goda, S.]] | [[Category: Goda, S.]] | ||
| - | [[Category: Mcleod, J | + | [[Category: Mcleod, J D.]] |
| - | [[Category: Papageorgiou, A | + | [[Category: Papageorgiou, A C.]] |
| - | [[Category: Sansom, D | + | [[Category: Sansom, D M.]] |
| - | [[Category: Tranter, H | + | [[Category: Tranter, H S.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: enterotoxin]] | [[Category: enterotoxin]] | ||
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[[Category: zinc-binding site]] | [[Category: zinc-binding site]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:26:30 2008'' |
Revision as of 13:26, 21 February 2008
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IDENTIFICATION OF A SECONDARY ZINC-BINDING SITE IN STAPHYLOCOCCAL ENTEROTOXIN C2: IMPLICATIONS FOR SUPERANTIGEN RECOGNITION
Overview
The previously determined crystal structure of the superantigen staphylococcal enterotoxin C2 (SEC2) showed binding of a single zinc ion located between the N- and C-terminal domains. Here we present the crystal structure of SEC2 determined to 2.0 A resolution in the presence of additional zinc. The structure revealed the presence of a secondary zinc-binding site close to the major histocompatibility complex (MHC)-binding site of the toxin and some 28 A away from the primary zinc-binding site of the toxin found in previous studies. T cell stimulation assays showed that varying the concentration of zinc ions present affected the activity of the toxin and we observed that high zinc concentrations considerably inhibited T cell responses. This indicates that SEC2 may have multiple modes of interaction with the immune system that are dependent on serum zinc levels. The potential role of the secondary zinc-binding site and that of the primary one in the formation of the TCR.SEC2.MHC complex are considered, and the possibility that zinc may regulate the activity of SEC2 as a toxin facilitating different T cell responses is discussed.
About this Structure
1UNS is a Single protein structure of sequence from Staphylococcus aureus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Identification of a secondary zinc-binding site in staphylococcal enterotoxin C2. Implications for superantigen recognition., Papageorgiou AC, Baker MD, McLeod JD, Goda SK, Manzotti CN, Sansom DM, Tranter HS, Acharya KR, J Biol Chem. 2004 Jan 9;279(2):1297-303. Epub 2003 Oct 14. PMID:14559915
Page seeded by OCA on Thu Feb 21 15:26:30 2008
