1gpi
From Proteopedia
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Revision as of 13:11, 30 October 2007
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CELLOBIOHYDROLASE CEL7D (CBH 58) FROM PHANEROCHAETE CHRYSOSPORIUM. CATALYTIC MODULE AT 1.32 ANG RESOLUTION
Overview
Cellobiohydrolase 58 (Cel7D) is the major cellulase produced by the, white-rot fungus Phanerochaete chrysosporium, constituting approximately, 10 % of the total secreted protein in liquid culture on cellulose. The, enzyme is classified into family 7 of the glycosyl hydrolases, together, with cellobiohydrolase I (Cel7A) and endoglucanase I (Cel7B) from, Trichoderma reesei. Like those enzymes, it catalyses cellulose hydrolysis, with net retention of the anomeric carbon configuration.The structure of, the catalytic module (431 residues) of Cel7D was determined at 3.0 A, resolution using the structure of Cel7A from T. reesei as a search model, in molecular replacement, and ultimately refined at 1.32 A resolution. The, core structure is a beta-sandwich composed of two large and mainly, ... [(full description)]
About this Structure
1GPI is a [Single protein] structure of sequence from [Phanerochaete chrysosporium] with NAG as [ligand]. Active as [Cellulose 1,4-beta-cellobiosidase], with EC number [3.2.1.91]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
Family 7 cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 A resolution and homology models of the isozymes., Munoz IG, Ubhayasekera W, Henriksson H, Szabo I, Pettersson G, Johansson G, Mowbray SL, Stahlberg J, J Mol Biol. 2001 Dec 14;314(5):1097-111. PMID:11743726
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