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1uon
From Proteopedia
(New page: 200px<br /><applet load="1uon" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uon" /> '''REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY EL...) |
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| - | [[Image:1uon.gif|left|200px]]<br /><applet load="1uon" size=" | + | [[Image:1uon.gif|left|200px]]<br /><applet load="1uon" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uon" /> | caption="1uon" /> | ||
'''REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION'''<br /> | '''REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Reovirus is an icosahedral, double-stranded (ds) RNA virus that uses viral | + | Reovirus is an icosahedral, double-stranded (ds) RNA virus that uses viral polymerases packaged within the viral core to transcribe its ten distinct plus-strand RNAs. To localize these polymerases, the structure of the reovirion was refined to a resolution of 7.6 A by cryo-electron microscopy (cryo-EM) and three-dimensional (3D) image reconstruction. X-ray crystal models of reovirus proteins, including polymerase lambda 3, were then fitted into the density map. Each copy of lambda 3 was found anchored to the inner surface of the icosahedral core shell, making major contacts with three molecules of shell protein lambda 1 and overlapping, but not centering on, a five-fold axis. The overlap explains why only one copy of lambda 3 is bound per vertex. lambda 3 is furthermore oriented with its transcript exit channel facing a small channel through the lambda 1 shell, suggesting how the nascent RNA is passed into the large external cavity of the pentameric capping enzyme complex formed by protein lambda 2. |
==About this Structure== | ==About this Structure== | ||
| - | 1UON is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.] with MN and CH1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1UON is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CH1:'>CH1</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UON OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Reovirus sp.]] | [[Category: Reovirus sp.]] | ||
| - | [[Category: Baker, T | + | [[Category: Baker, T S.]] |
| - | [[Category: Chipman, P | + | [[Category: Chipman, P R.]] |
| - | [[Category: Nibert, M | + | [[Category: Nibert, M L.]] |
| - | [[Category: Walker, S | + | [[Category: Walker, S B.]] |
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
[[Category: CH1]] | [[Category: CH1]] | ||
| Line 25: | Line 25: | ||
[[Category: reovirus]] | [[Category: reovirus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:26:46 2008'' |
Revision as of 13:26, 21 February 2008
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REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION
Overview
Reovirus is an icosahedral, double-stranded (ds) RNA virus that uses viral polymerases packaged within the viral core to transcribe its ten distinct plus-strand RNAs. To localize these polymerases, the structure of the reovirion was refined to a resolution of 7.6 A by cryo-electron microscopy (cryo-EM) and three-dimensional (3D) image reconstruction. X-ray crystal models of reovirus proteins, including polymerase lambda 3, were then fitted into the density map. Each copy of lambda 3 was found anchored to the inner surface of the icosahedral core shell, making major contacts with three molecules of shell protein lambda 1 and overlapping, but not centering on, a five-fold axis. The overlap explains why only one copy of lambda 3 is bound per vertex. lambda 3 is furthermore oriented with its transcript exit channel facing a small channel through the lambda 1 shell, suggesting how the nascent RNA is passed into the large external cavity of the pentameric capping enzyme complex formed by protein lambda 2.
About this Structure
1UON is a Protein complex structure of sequences from Reovirus sp. with and as ligands. Full crystallographic information is available from OCA.
Reference
Reovirus polymerase lambda 3 localized by cryo-electron microscopy of virions at a resolution of 7.6 A., Zhang X, Walker SB, Chipman PR, Nibert ML, Baker TS, Nat Struct Biol. 2003 Dec;10(12):1011-8. Epub 2003 Nov 9. PMID:14608373
Page seeded by OCA on Thu Feb 21 15:26:46 2008
Categories: Protein complex | Reovirus sp. | Baker, T S. | Chipman, P R. | Nibert, M L. | Walker, S B. | Zhang, X. | CH1 | MN | Core protein | Cryoem | Polymerase | Reovirus
