1urj
From Proteopedia
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==Overview== | ==Overview== | ||
| - | All organisms including animal viruses use specific proteins to bind | + | All organisms including animal viruses use specific proteins to bind single-stranded DNA rapidly in a non-sequence-specific, flexible, and cooperative manner during the DNA replication process. The crystal structure of a 60-residue C-terminal deletion construct of ICP8, the major single-stranded DNA-binding protein from herpes simplex virus-1, was determined at 3.0 A resolution. The structure reveals a novel fold, consisting of a large N-terminal domain (residues 9-1038) and a small C-terminal domain (residues 1049-1129). On the basis of the structure and the nearest neighbor interactions in the crystal, we have presented a model describing the site of single-stranded DNA binding and explaining the basis for cooperative binding. This model agrees with the beaded morphology observed in electron micrographs. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Mapelli, M.]] | [[Category: Mapelli, M.]] | ||
[[Category: Panjikar, S.]] | [[Category: Panjikar, S.]] | ||
| - | [[Category: Tucker, P | + | [[Category: Tucker, P A.]] |
[[Category: HG]] | [[Category: HG]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:27:31 2008'' |
Revision as of 13:27, 21 February 2008
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SINGLE STRANDED DNA-BINDING PROTEIN(ICP8) FROM HERPES SIMPLEX VIRUS-1
Overview
All organisms including animal viruses use specific proteins to bind single-stranded DNA rapidly in a non-sequence-specific, flexible, and cooperative manner during the DNA replication process. The crystal structure of a 60-residue C-terminal deletion construct of ICP8, the major single-stranded DNA-binding protein from herpes simplex virus-1, was determined at 3.0 A resolution. The structure reveals a novel fold, consisting of a large N-terminal domain (residues 9-1038) and a small C-terminal domain (residues 1049-1129). On the basis of the structure and the nearest neighbor interactions in the crystal, we have presented a model describing the site of single-stranded DNA binding and explaining the basis for cooperative binding. This model agrees with the beaded morphology observed in electron micrographs.
About this Structure
1URJ is a Single protein structure of sequence from Human herpesvirus 4 with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding., Mapelli M, Panjikar S, Tucker PA, J Biol Chem. 2005 Jan 28;280(4):2990-7. Epub 2004 Oct 26. PMID:15507432
Page seeded by OCA on Thu Feb 21 15:27:31 2008
Categories: Human herpesvirus 4 | Single protein | Mapelli, M. | Panjikar, S. | Tucker, P A. | HG | ZN | Dna replication | Dna-binding | Hsv-1 | Icp8 | Nuclear protein. | Ssb | Zinc-finger
