1uus
From Proteopedia
(New page: 200px<br /><applet load="1uus" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uus, resolution 2.8Å" /> '''STRUCTURE OF AN ACTIV...) |
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| - | [[Image:1uus.jpg|left|200px]]<br /><applet load="1uus" size=" | + | [[Image:1uus.jpg|left|200px]]<br /><applet load="1uus" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uus, resolution 2.8Å" /> | caption="1uus, resolution 2.8Å" /> | ||
'''STRUCTURE OF AN ACTIVATED DICTYOSTELIUM STAT IN ITS DNA-UNBOUND FORM'''<br /> | '''STRUCTURE OF AN ACTIVATED DICTYOSTELIUM STAT IN ITS DNA-UNBOUND FORM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Dd-STATa is a STAT protein which transcriptionally regulates cellular | + | Dd-STATa is a STAT protein which transcriptionally regulates cellular differentiation in Dictyostelium discoideum, the only non-metazoan known to employ SH2 domain signaling. The 2.7 A crystal structure of a tyrosine phosphorylated Dd-STATa homodimer reveals a four-domain architecture similar to that of mammalian STATs 1 and 3, but with an inverted orientation for the coiled-coil domain. Dimerization is mediated by SH2 domain:phosphopeptide interactions and by a direct interaction between SH2 domains. The unliganded Dd-STATa dimer adopts a fully extended conformation remarkably different from that of the DNA-bound mammalian STATs, implying a large conformational change upon target site recognition. Buried hydrophilic residues predicted to destabilize the coiled-coil domain suggest how hydrophobic residues may become exposed and mediate nuclear export. Functional and evolutionary implications for metazoan STAT proteins are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1UUS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http:// | + | 1UUS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UUS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Fukuzawa, M.]] | [[Category: Fukuzawa, M.]] | ||
| - | [[Category: Muller, C | + | [[Category: Muller, C W.]] |
[[Category: Petosa, C.]] | [[Category: Petosa, C.]] | ||
[[Category: Ravelli, R.]] | [[Category: Ravelli, R.]] | ||
[[Category: Soler-Lopez, M.]] | [[Category: Soler-Lopez, M.]] | ||
| - | [[Category: Williams, J | + | [[Category: Williams, J G.]] |
[[Category: dictyostelium]] | [[Category: dictyostelium]] | ||
[[Category: sh2]] | [[Category: sh2]] | ||
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[[Category: transducer]] | [[Category: transducer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:28:32 2008'' |
Revision as of 13:28, 21 February 2008
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STRUCTURE OF AN ACTIVATED DICTYOSTELIUM STAT IN ITS DNA-UNBOUND FORM
Overview
Dd-STATa is a STAT protein which transcriptionally regulates cellular differentiation in Dictyostelium discoideum, the only non-metazoan known to employ SH2 domain signaling. The 2.7 A crystal structure of a tyrosine phosphorylated Dd-STATa homodimer reveals a four-domain architecture similar to that of mammalian STATs 1 and 3, but with an inverted orientation for the coiled-coil domain. Dimerization is mediated by SH2 domain:phosphopeptide interactions and by a direct interaction between SH2 domains. The unliganded Dd-STATa dimer adopts a fully extended conformation remarkably different from that of the DNA-bound mammalian STATs, implying a large conformational change upon target site recognition. Buried hydrophilic residues predicted to destabilize the coiled-coil domain suggest how hydrophobic residues may become exposed and mediate nuclear export. Functional and evolutionary implications for metazoan STAT proteins are discussed.
About this Structure
1UUS is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.
Reference
Structure of an activated Dictyostelium STAT in its DNA-unbound form., Soler-Lopez M, Petosa C, Fukuzawa M, Ravelli R, Williams JG, Muller CW, Mol Cell. 2004 Mar 26;13(6):791-804. PMID:15053873
Page seeded by OCA on Thu Feb 21 15:28:32 2008
