1v2z
From Proteopedia
(New page: 200px<br /><applet load="1v2z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v2z, resolution 1.80Å" /> '''Crystal structure of...) |
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| - | [[Image:1v2z.jpg|left|200px]]<br /><applet load="1v2z" size=" | + | [[Image:1v2z.jpg|left|200px]]<br /><applet load="1v2z" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1v2z, resolution 1.80Å" /> | caption="1v2z, resolution 1.80Å" /> | ||
'''Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA'''<br /> | '''Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | KaiA, KaiB and KaiC constitute the circadian clock machinery in | + | KaiA, KaiB and KaiC constitute the circadian clock machinery in cyanobacteria, and KaiA activates kaiBC expression whereas KaiC represses it. Here we show that KaiA is composed of three functional domains, the N-terminal amplitude-amplifier domain, the central period-adjuster domain and the C-terminal clock-oscillator domain. The C-terminal domain is responsible for dimer formation, binding to KaiC, enhancing KaiC phosphorylation and generating the circadian oscillations. The X-ray crystal structure at a resolution of 1.8 A of the C-terminal clock-oscillator domain of KaiA from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 shows that residue His270, located at the center of a KaiA dimer concavity, is essential to KaiA function. KaiA binding to KaiC probably occurs via the concave surface. On the basis of the structure, we predict the structural roles of the residues that affect circadian oscillations. |
==About this Structure== | ==About this Structure== | ||
| - | 1V2Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http:// | + | 1V2Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kato, H.]] | [[Category: Kato, H.]] | ||
[[Category: Nakatsu, T.]] | [[Category: Nakatsu, T.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Shibata, H.]] | [[Category: Shibata, H.]] | ||
[[Category: Uzumaki, T.]] | [[Category: Uzumaki, T.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:30:58 2008'' |
Revision as of 13:31, 21 February 2008
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Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA
Overview
KaiA, KaiB and KaiC constitute the circadian clock machinery in cyanobacteria, and KaiA activates kaiBC expression whereas KaiC represses it. Here we show that KaiA is composed of three functional domains, the N-terminal amplitude-amplifier domain, the central period-adjuster domain and the C-terminal clock-oscillator domain. The C-terminal domain is responsible for dimer formation, binding to KaiC, enhancing KaiC phosphorylation and generating the circadian oscillations. The X-ray crystal structure at a resolution of 1.8 A of the C-terminal clock-oscillator domain of KaiA from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 shows that residue His270, located at the center of a KaiA dimer concavity, is essential to KaiA function. KaiA binding to KaiC probably occurs via the concave surface. On the basis of the structure, we predict the structural roles of the residues that affect circadian oscillations.
About this Structure
1V2Z is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.
Reference
Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein., Uzumaki T, Fujita M, Nakatsu T, Hayashi F, Shibata H, Itoh N, Kato H, Ishiura M, Nat Struct Mol Biol. 2004 Jul;11(7):623-31. Epub 2004 May 30. PMID:15170179
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