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1gt6

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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:18:04 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:18:42 2007''

Revision as of 13:13, 30 October 2007

Image:1gt6.gif

1gt6, resolution 2.2Å

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S146A MUTANT OF THERMOMYCES (HUMICOLA) LANUGINOSA LIPASE COMPLEX WITH OLEIC ACID

Overview

The binding of Thermomyces lanuginosa lipase and its mutants [TLL(S146A), TLL(W89L), TLL(W117F, W221H, W260H)] to the mixed micelles of, cis-parinaric acid/sodium taurodeoxycholate at pH 5.0 led to the quenching, of the intrinsic tryptophan fluorescence emission (300-380 nm) and to a, simultaneous increase in the cis-parinaric acid fluorescence emission, (380-500 nm). These findings were used to characterize the Thermomyces, lanuginosa lipase/cis-parinaric acid interactions occurring in the, presence of sodium taurodeoxycholate.The fluorescence resonance energy, transfer and Stern-Volmer quenching constant values obtained were, correlated with the accessibility of the tryptophan residues to the, cis-parinaric acid and with the lid opening ability of Thermomyces, lanuginosa lipase (and its ... [(full description)]

About this Structure

1GT6 is a [Single protein] structure of sequence from [Thermomyces lanuginosus] with OLA as [ligand]. Active as [Triacylglycerol lipase], with EC number [3.1.1.3]. Structure known Active Site: OLA. Full crystallographic information is available from [OCA].

Reference

Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC., Yapoudjian S, Ivanova MG, Brzozowski AM, Patkar SA, Vind J, Svendsen A, Verger R, Eur J Biochem. 2002 Mar;269(6):1613-21. PMID:11895431

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