1v7x
From Proteopedia
(New page: 200px<br /><applet load="1v7x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v7x, resolution 2.00Å" /> '''Crystal structure of...) |
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| - | [[Image:1v7x.jpg|left|200px]]<br /><applet load="1v7x" size=" | + | [[Image:1v7x.jpg|left|200px]]<br /><applet load="1v7x" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1v7x, resolution 2.00Å" /> | caption="1v7x, resolution 2.00Å" /> | ||
'''Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate'''<br /> | '''Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Vibrio proteolyticus chitobiose phosphorylase (ChBP) belongs to glycosyl | + | Vibrio proteolyticus chitobiose phosphorylase (ChBP) belongs to glycosyl transferase family 36 (GT-36), and catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. As the first known structures of a GT-36 enzyme, we determined the crystal structure of ChBP in a ternary complex with GlcNAc and SO(4). It is also the first structures of an inverting phosphorolytic enzyme in a complex with a sugar and a sulfate ion, and reveals a pseudo-ternary complex structure of enzyme-sugar-phosphate. ChBP comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain, constituting a distinctive structure among GT families. Instead, it shows significant structural similarity with glycoside hydrolase (GH) enzymes, glucoamylases (GH-15), and maltose phosphorylase (GH-65) in clan GH-L. The structural similarity reported here, together with distant sequence similarities between ChBP and GHs, led to the reclassification of family GT-36 into a novel GH family, namely GH-94. |
==About this Structure== | ==About this Structure== | ||
| - | 1V7X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus] with NDG, NAG, CA and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1V7X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus] with <scene name='pdbligand=NDG:'>NDG</scene>, <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: beta-sandwich]] | [[Category: beta-sandwich]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:32:28 2008'' |
Revision as of 13:32, 21 February 2008
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Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate
Overview
Vibrio proteolyticus chitobiose phosphorylase (ChBP) belongs to glycosyl transferase family 36 (GT-36), and catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. As the first known structures of a GT-36 enzyme, we determined the crystal structure of ChBP in a ternary complex with GlcNAc and SO(4). It is also the first structures of an inverting phosphorolytic enzyme in a complex with a sugar and a sulfate ion, and reveals a pseudo-ternary complex structure of enzyme-sugar-phosphate. ChBP comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain, constituting a distinctive structure among GT families. Instead, it shows significant structural similarity with glycoside hydrolase (GH) enzymes, glucoamylases (GH-15), and maltose phosphorylase (GH-65) in clan GH-L. The structural similarity reported here, together with distant sequence similarities between ChBP and GHs, led to the reclassification of family GT-36 into a novel GH family, namely GH-94.
About this Structure
1V7X is a Single protein structure of sequence from Vibrio proteolyticus with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold., Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, Fushinobu S, Structure. 2004 Jun;12(6):937-47. PMID:15274915
Page seeded by OCA on Thu Feb 21 15:32:28 2008
Categories: Single protein | Vibrio proteolyticus | Fushinobu, S. | Hayashi, K. | Hidaka, M. | Honda, Y. | Kitaoka, M. | Nirasawa, S. | Shoun, H. | Wakagi, T. | CA | NAG | NDG | SO4 | (alpha/alpha)6 barrel | Beta-sandwich
