1v9g

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1v9g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v9g, resolution 1.8&Aring;" /> '''Neutron Crystallograp...)
Line 1: Line 1:
-
[[Image:1v9g.gif|left|200px]]<br /><applet load="1v9g" size="450" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1v9g.gif|left|200px]]<br /><applet load="1v9g" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1v9g, resolution 1.8&Aring;" />
caption="1v9g, resolution 1.8&Aring;" />
'''Neutron Crystallographic analysis of the Z-DNA hexamer CGCGCG'''<br />
'''Neutron Crystallographic analysis of the Z-DNA hexamer CGCGCG'''<br />
==Overview==
==Overview==
-
In order to reveal the hydration structure of Z-DNA, a neutron diffraction, study has been carried out at 1.8 A resolution on a Z-DNA hexamer, d(CGCGCG). Neutron diffraction data were collected with the BIX-3, single-crystal diffractometer at the JRR-3 reactor in the Japan Atomic, Energy Research Institute (JAERI) using a large crystal (1.6 mm3) obtained, from D2O solution. It has been found that almost all the guanine bases, have participated in H/D exchange at the C8-H8 group, consistent with the, acidic nature of this bond. 44 water molecules were found in the nuclear, density maps, of which 29 showed the entire contour of all three atoms, (D-O-D). The remaining 15 water molecules had a simple spherical shape, indicating that they were rotationally disordered. An interesting, relationship was found between the orientational disorder of the water, molecules and their locations. Almost all water molecules in the minor, groove were well ordered in the crystal, while 40% of the water molecules, in the major groove were rotationally disordered. The hydrogen-bonding, networks in the hydration shells have two structural aspects: flexibility, and regularity.
+
In order to reveal the hydration structure of Z-DNA, a neutron diffraction study has been carried out at 1.8 A resolution on a Z-DNA hexamer d(CGCGCG). Neutron diffraction data were collected with the BIX-3 single-crystal diffractometer at the JRR-3 reactor in the Japan Atomic Energy Research Institute (JAERI) using a large crystal (1.6 mm3) obtained from D2O solution. It has been found that almost all the guanine bases have participated in H/D exchange at the C8-H8 group, consistent with the acidic nature of this bond. 44 water molecules were found in the nuclear density maps, of which 29 showed the entire contour of all three atoms (D-O-D). The remaining 15 water molecules had a simple spherical shape, indicating that they were rotationally disordered. An interesting relationship was found between the orientational disorder of the water molecules and their locations. Almost all water molecules in the minor groove were well ordered in the crystal, while 40% of the water molecules in the major groove were rotationally disordered. The hydrogen-bonding networks in the hydration shells have two structural aspects: flexibility and regularity.
==About this Structure==
==About this Structure==
-
1V9G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPW and DOD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V9G OCA].
+
1V9G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPW:'>SPW</scene> and <scene name='pdbligand=DOD:'>DOD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9G OCA].
==Reference==
==Reference==
Line 23: Line 23:
[[Category: z-dna]]
[[Category: z-dna]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:55:08 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:32:55 2008''

Revision as of 13:32, 21 February 2008

Image:1v9g.gif

1v9g, resolution 1.8Å

Drag the structure with the mouse to rotate

Neutron Crystallographic analysis of the Z-DNA hexamer CGCGCG

Overview

In order to reveal the hydration structure of Z-DNA, a neutron diffraction study has been carried out at 1.8 A resolution on a Z-DNA hexamer d(CGCGCG). Neutron diffraction data were collected with the BIX-3 single-crystal diffractometer at the JRR-3 reactor in the Japan Atomic Energy Research Institute (JAERI) using a large crystal (1.6 mm3) obtained from D2O solution. It has been found that almost all the guanine bases have participated in H/D exchange at the C8-H8 group, consistent with the acidic nature of this bond. 44 water molecules were found in the nuclear density maps, of which 29 showed the entire contour of all three atoms (D-O-D). The remaining 15 water molecules had a simple spherical shape, indicating that they were rotationally disordered. An interesting relationship was found between the orientational disorder of the water molecules and their locations. Almost all water molecules in the minor groove were well ordered in the crystal, while 40% of the water molecules in the major groove were rotationally disordered. The hydrogen-bonding networks in the hydration shells have two structural aspects: flexibility and regularity.

About this Structure

1V9G is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.

Reference

The hydration structure of a Z-DNA hexameric duplex determined by a neutron diffraction technique., Chatake T, Tanaka I, Umino H, Arai S, Niimura N, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1088-98. Epub 2005, Jul 20. PMID:16041074

Page seeded by OCA on Thu Feb 21 15:32:55 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1v9g"
Personal tools