1vgl
From Proteopedia
(New page: 200px<br /><applet load="1vgl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vgl, resolution 2.6Å" /> '''Crystal structure of ...) |
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| - | [[Image:1vgl.gif|left|200px]]<br /><applet load="1vgl" size=" | + | [[Image:1vgl.gif|left|200px]]<br /><applet load="1vgl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vgl, resolution 2.6Å" /> | caption="1vgl, resolution 2.6Å" /> | ||
'''Crystal structure of tetrameric KaiB from T.elongatus BP-1'''<br /> | '''Crystal structure of tetrameric KaiB from T.elongatus BP-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | KaiB is a component of the circadian clock oscillator in cyanobacteria, which are the simplest organisms that exhibit circadian rhythms. KaiB | + | KaiB is a component of the circadian clock oscillator in cyanobacteria, which are the simplest organisms that exhibit circadian rhythms. KaiB consists of 108 amino-acid residues and has a molecular weight of 12 025 Da. KaiB and Cys-substituted KaiB mutants from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 were expressed as GST-fusion proteins in Escherichia coli, purified and crystallized. The crystals of wild-type KaiB belong to the monoclinic space group P2(1), with unit-cell parameters a = 89.6, b = 71.2, c = 106.8 A, beta = 100.1 degrees. While the native crystals diffract to 3.7 A, osmium derivatives, which show an approximately 4 A shrinkage in the b axis, diffract to 2.6 A. The crystals of the singly Cys-substituted mutant T64C with Hg, which show different morphology, diffract to 2.5 A and belong to the monoclinic space group P2, with unit-cell parameters a = 63.7, b = 33.4, c = 93.7 A, beta = 100.1 degrees. Anomalous difference Patterson maps of the Os- and Hg-derivative crystals had significant peaks in their Harker sections, suggesting that both derivatives are suitable for structure determination. |
==About this Structure== | ==About this Structure== | ||
| - | 1VGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1VGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria] with <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VGL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: circadian clock protein]] | [[Category: circadian clock protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:35:02 2008'' |
Revision as of 13:35, 21 February 2008
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Crystal structure of tetrameric KaiB from T.elongatus BP-1
Overview
KaiB is a component of the circadian clock oscillator in cyanobacteria, which are the simplest organisms that exhibit circadian rhythms. KaiB consists of 108 amino-acid residues and has a molecular weight of 12 025 Da. KaiB and Cys-substituted KaiB mutants from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 were expressed as GST-fusion proteins in Escherichia coli, purified and crystallized. The crystals of wild-type KaiB belong to the monoclinic space group P2(1), with unit-cell parameters a = 89.6, b = 71.2, c = 106.8 A, beta = 100.1 degrees. While the native crystals diffract to 3.7 A, osmium derivatives, which show an approximately 4 A shrinkage in the b axis, diffract to 2.6 A. The crystals of the singly Cys-substituted mutant T64C with Hg, which show different morphology, diffract to 2.5 A and belong to the monoclinic space group P2, with unit-cell parameters a = 63.7, b = 33.4, c = 93.7 A, beta = 100.1 degrees. Anomalous difference Patterson maps of the Os- and Hg-derivative crystals had significant peaks in their Harker sections, suggesting that both derivatives are suitable for structure determination.
About this Structure
1VGL is a Single protein structure of sequence from Bacteria with as ligand. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1., Iwase R, Imada K, Hayashi F, Uzumaki T, Namba K, Ishiura M, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):727-9. Epub 2004, Mar 23. PMID:15039567
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