1vm1
From Proteopedia
(New page: 200px<br /><applet load="1vm1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vm1, resolution 2.02Å" /> '''STRUCTURE OF SHV-1 B...) |
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| - | [[Image:1vm1.gif|left|200px]]<br /><applet load="1vm1" size=" | + | [[Image:1vm1.gif|left|200px]]<br /><applet load="1vm1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vm1, resolution 2.02Å" /> | caption="1vm1, resolution 2.02Å" /> | ||
'''STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM'''<br /> | '''STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Two species resulting from the reaction of the SHV-1 class A | + | Two species resulting from the reaction of the SHV-1 class A beta-lactamase with the sulfone inhibitor tazobactam have been trapped at 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of tazobactam, is bonded to Ser130. It is proposed that the electron density map of the crystal is a composite picture of two complexes, each with only a single bound species. It is estimated that the two complexes exist in the crystal in approximately equal populations. Results are discussed in relation to the mechanism-based inhibition of class A beta-lactamases by the similar inhibitors sulbactam and clavulanic acid. |
==About this Structure== | ==About this Structure== | ||
| - | 1VM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with MA4, TBE, AKR and TAZ as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | + | 1VM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with <scene name='pdbligand=MA4:'>MA4</scene>, <scene name='pdbligand=TBE:'>TBE</scene>, <scene name='pdbligand=AKR:'>AKR</scene> and <scene name='pdbligand=TAZ:'>TAZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1G56. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VM1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Klebsiella pneumoniae]] | [[Category: Klebsiella pneumoniae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bonomo, R | + | [[Category: Bonomo, R A.]] |
[[Category: Hujer, A.]] | [[Category: Hujer, A.]] | ||
| - | [[Category: Knox, J | + | [[Category: Knox, J R.]] |
| - | [[Category: Kuzin, A | + | [[Category: Kuzin, A P.]] |
[[Category: Nukaga, M.]] | [[Category: Nukaga, M.]] | ||
[[Category: Nukaga, Y.]] | [[Category: Nukaga, Y.]] | ||
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[[Category: penicillinase]] | [[Category: penicillinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:36:40 2008'' |
Revision as of 13:36, 21 February 2008
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STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM
Overview
Two species resulting from the reaction of the SHV-1 class A beta-lactamase with the sulfone inhibitor tazobactam have been trapped at 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of tazobactam, is bonded to Ser130. It is proposed that the electron density map of the crystal is a composite picture of two complexes, each with only a single bound species. It is estimated that the two complexes exist in the crystal in approximately equal populations. Results are discussed in relation to the mechanism-based inhibition of class A beta-lactamases by the similar inhibitors sulbactam and clavulanic acid.
About this Structure
1VM1 is a Single protein structure of sequence from Klebsiella pneumoniae with , , and as ligands. This structure supersedes the now removed PDB entry 1G56. Active as Beta-lactamase, with EC number 3.5.2.6 Full crystallographic information is available from OCA.
Reference
Inhibition of the SHV-1 beta-lactamase by sulfones: crystallographic observation of two reaction intermediates with tazobactam., Kuzin AP, Nukaga M, Nukaga Y, Hujer A, Bonomo RA, Knox JR, Biochemistry. 2001 Feb 13;40(6):1861-6. PMID:11327849
Page seeded by OCA on Thu Feb 21 15:36:40 2008
Categories: Beta-lactamase | Klebsiella pneumoniae | Single protein | Bonomo, R A. | Hujer, A. | Knox, J R. | Kuzin, A P. | Nukaga, M. | Nukaga, Y. | AKR | MA4 | TAZ | TBE | Beta-lactam hydrolase | Detergent binding | Inhibitor design | Penicillinase
