1vox
From Proteopedia
(New page: 200px<br /><applet load="1vox" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vox, resolution 11.5Å" /> '''Crystal structure of...) |
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| - | [[Image:1vox.gif|left|200px]]<br /><applet load="1vox" size=" | + | [[Image:1vox.gif|left|200px]]<br /><applet load="1vox" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vox, resolution 11.5Å" /> | caption="1vox, resolution 11.5Å" /> | ||
'''Crystal structure of five 70s ribosomes from Escherichia Coli in complex with protein Y. This file contains the 30s subunit of one 70s ribosome. The entire crystal structure contains five 70s ribosomes and is described in remark 400.'''<br /> | '''Crystal structure of five 70s ribosomes from Escherichia Coli in complex with protein Y. This file contains the 30s subunit of one 70s ribosome. The entire crystal structure contains five 70s ribosomes and is described in remark 400.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | During environmental stress, organisms limit protein synthesis by storing | + | During environmental stress, organisms limit protein synthesis by storing inactive ribosomes that are rapidly reactivated when conditions improve. Here we present structural and biochemical data showing that protein Y, an Escherichia coli stress protein, fills the tRNA- and mRNA-binding channel of the small ribosomal subunit to stabilize intact ribosomes. Protein Y inhibits translation initiation during cold shock but not at normal temperatures. Furthermore, protein Y competes with conserved translation initiation factors that, in bacteria, are required for ribosomal subunit dissociation. The mechanism used by protein Y to reduce translation initiation during stress and quickly release ribosomes for renewed translation initiation may therefore occur widely in nature. |
==About this Structure== | ==About this Structure== | ||
| - | 1VOX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1VOX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VOX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cate, J | + | [[Category: Cate, J H.]] |
| - | [[Category: Hau, C | + | [[Category: Hau, C W.]] |
| - | [[Category: Schuwirth, B | + | [[Category: Schuwirth, B S.]] |
[[Category: Vila-Sanjurjo, A.]] | [[Category: Vila-Sanjurjo, A.]] | ||
[[Category: 30s ribosomal subunit]] | [[Category: 30s ribosomal subunit]] | ||
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[[Category: rna-rna complex]] | [[Category: rna-rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:37:05 2008'' |
Revision as of 13:37, 21 February 2008
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Crystal structure of five 70s ribosomes from Escherichia Coli in complex with protein Y. This file contains the 30s subunit of one 70s ribosome. The entire crystal structure contains five 70s ribosomes and is described in remark 400.
Overview
During environmental stress, organisms limit protein synthesis by storing inactive ribosomes that are rapidly reactivated when conditions improve. Here we present structural and biochemical data showing that protein Y, an Escherichia coli stress protein, fills the tRNA- and mRNA-binding channel of the small ribosomal subunit to stabilize intact ribosomes. Protein Y inhibits translation initiation during cold shock but not at normal temperatures. Furthermore, protein Y competes with conserved translation initiation factors that, in bacteria, are required for ribosomal subunit dissociation. The mechanism used by protein Y to reduce translation initiation during stress and quickly release ribosomes for renewed translation initiation may therefore occur widely in nature.
About this Structure
1VOX is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis for the control of translation initiation during stress., Vila-Sanjurjo A, Schuwirth BS, Hau CW, Cate JH, Nat Struct Mol Biol. 2004 Nov;11(11):1054-9. Epub 2004 Oct 24. PMID:15502846
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