1gwd

From Proteopedia

Revision as of 13:16, 30 October 2007

TRI-IODIDE DERIVATIVE OF HEN EGG-WHITE LYSOZYME

Overview

A series of experiments performed at Cu Kalpha wavelength on in-house, X-ray equipment are presented which investigate two possibilities for, enhancing the experimental phasing signal by means of (i) triiodide/iodide, soaks using KI/I(2) and (ii) combinations of counter-ions introduced using, the short cryosoak method. Triiodide-derivative crystal structures for, five test proteins have been refined and reveal that iodine can bind as, polyiodide and single iodide ions through hydrophobic and hydrogen-bonding, interactions both at the molecular surface and in intramolecular and, intermolecular cavities. In three cases, the structures could be, automatically determined with autoSHARP using in-house SAD and SIRAS data., The investigation of combinatorial counter-ion replacement using ... [(full description)]

About this Structure

1GWD is a [Single protein] structure of sequence from [Gallus gallus] with IOD, CL, NA, EDO and CMO as [ligands]. Active as [Lysozyme], with EC number [3.2.1.17]. Structure known Active Site: EA1. Full crystallographic information is available from [OCA].

Reference

Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:12037300

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