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1vzo
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Mitogen and stress-activated kinase-1 (MSK1) is a serine/threonine protein | + | Mitogen and stress-activated kinase-1 (MSK1) is a serine/threonine protein kinase that is activated by either p38 or p42ERK MAPKs in response to stress or mitogenic extracellular stimuli. MSK1 belongs to a family of protein kinases that contain two distinct kinase domains in one polypeptide chain. We report the 1.8 A crystal structure of the N-terminal kinase domain of MSK1. The crystal structure reveals a unique inactive conformation with the ATP binding site blocked by the nucleotide binding loop. This inactive conformation is stabilized by the formation of a new three-stranded beta sheet on the N lobe of the kinase domain. The three beta strands come from residues at the N terminus of the kinase domain, what would be the alphaB helix in the active conformation, and the activation loop. The new three-stranded beta sheet occupies a position equivalent to the N terminus of the alphaC helix in active protein kinases. |
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Transferred entry: 2 | + | [[Category: Transferred entry: 2 7.11 1]] |
[[Category: Bax, B.]] | [[Category: Bax, B.]] | ||
[[Category: Bridges, A.]] | [[Category: Bridges, A.]] | ||
[[Category: Brown, M.]] | [[Category: Brown, M.]] | ||
| - | [[Category: Carter, P | + | [[Category: Carter, P S.]] |
[[Category: Clarke, A.]] | [[Category: Clarke, A.]] | ||
[[Category: Horrocks, P.]] | [[Category: Horrocks, P.]] | ||
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[[Category: Pettman, G.]] | [[Category: Pettman, G.]] | ||
[[Category: Reith, A.]] | [[Category: Reith, A.]] | ||
| - | [[Category: Smith, K | + | [[Category: Smith, K J.]] |
[[Category: Wilkinson, M.]] | [[Category: Wilkinson, M.]] | ||
[[Category: BME]] | [[Category: BME]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:39:01 2008'' |
Revision as of 13:39, 21 February 2008
|
THE STRUCTURE OF THE N-TERMINAL KINASE DOMAIN OF MSK1 REVEALS A NOVEL AUTOINHIBITORY CONFORMATION FOR A DUAL KINASE PROTEIN
Contents |
Overview
Mitogen and stress-activated kinase-1 (MSK1) is a serine/threonine protein kinase that is activated by either p38 or p42ERK MAPKs in response to stress or mitogenic extracellular stimuli. MSK1 belongs to a family of protein kinases that contain two distinct kinase domains in one polypeptide chain. We report the 1.8 A crystal structure of the N-terminal kinase domain of MSK1. The crystal structure reveals a unique inactive conformation with the ATP binding site blocked by the nucleotide binding loop. This inactive conformation is stabilized by the formation of a new three-stranded beta sheet on the N lobe of the kinase domain. The three beta strands come from residues at the N terminus of the kinase domain, what would be the alphaB helix in the active conformation, and the activation loop. The new three-stranded beta sheet occupies a position equivalent to the N terminus of the alphaC helix in active protein kinases.
Disease
Known disease associated with this structure: Spondyloepiphyseal dysplasia, Kimberley type OMIM:[155760]
About this Structure
1VZO is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Transferred entry: 2.7.11.1, with EC number 2.7.1.37 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structure of MSK1 reveals a novel autoinhibitory conformation for a dual kinase protein., Smith KJ, Carter PS, Bridges A, Horrocks P, Lewis C, Pettman G, Clarke A, Brown M, Hughes J, Wilkinson M, Bax B, Reith A, Structure. 2004 Jun;12(6):1067-77. PMID:15274926
Page seeded by OCA on Thu Feb 21 15:39:01 2008
Categories: Homo sapiens | Single protein | Transferred entry: 2 7.11 1 | Bax, B. | Bridges, A. | Brown, M. | Carter, P S. | Clarke, A. | Horrocks, P. | Hughes, J. | Lewis, C. | Pettman, G. | Reith, A. | Smith, K J. | Wilkinson, M. | BME | SO4 | Phosphorylation | Protein kinase | Serine/threonine protein kinase | Transferase
