1w31

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==Overview==
==Overview==
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The X-ray structure of the enzyme 5-aminolaevulinic acid dehydratase, (ALAD) from yeast complexed with the competitive inhibitor, 5-hydroxylaevulinic acid has been determined at a resolution of 1.9 A. The, structure shows that the inhibitor is bound by a Schiff-base link to one, of the invariant active-site lysine residues (Lys263). The inhibitor, appears to bind in two well defined conformations and the interactions, made by it suggest that it is a very close analogue of the substrate, 5-aminolaevulinic acid (ALA).
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The X-ray structure of the enzyme 5-aminolaevulinic acid dehydratase (ALAD) from yeast complexed with the competitive inhibitor 5-hydroxylaevulinic acid has been determined at a resolution of 1.9 A. The structure shows that the inhibitor is bound by a Schiff-base link to one of the invariant active-site lysine residues (Lys263). The inhibitor appears to bind in two well defined conformations and the interactions made by it suggest that it is a very close analogue of the substrate 5-aminolaevulinic acid (ALA).
==About this Structure==
==About this Structure==
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Beaven, G.D.E.]]
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[[Category: Beaven, G D.E.]]
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[[Category: Brindley, A.A.]]
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[[Category: Brindley, A A.]]
[[Category: Coates, L.]]
[[Category: Coates, L.]]
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[[Category: Cooper, J.B.]]
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[[Category: Cooper, J B.]]
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[[Category: Erskine, P.T.]]
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[[Category: Erskine, P T.]]
[[Category: Gill, R.]]
[[Category: Gill, R.]]
[[Category: Neier, R.]]
[[Category: Neier, R.]]
[[Category: Newbold, R.]]
[[Category: Newbold, R.]]
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[[Category: Shoolingin-Jordan, P.M.]]
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[[Category: Shoolingin-Jordan, P M.]]
[[Category: Stauffer, F.]]
[[Category: Stauffer, F.]]
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[[Category: Warren, M.J.]]
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[[Category: Warren, M J.]]
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[[Category: Wood, S.P.]]
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[[Category: Wood, S P.]]
[[Category: SHO]]
[[Category: SHO]]
[[Category: ZN]]
[[Category: ZN]]
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[[Category: zinc]]
[[Category: zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:18:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:39:55 2008''

Revision as of 13:39, 21 February 2008


1w31, resolution 1.9Å

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YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 5-HYDROXYLAEVULINIC ACID COMPLEX

Overview

The X-ray structure of the enzyme 5-aminolaevulinic acid dehydratase (ALAD) from yeast complexed with the competitive inhibitor 5-hydroxylaevulinic acid has been determined at a resolution of 1.9 A. The structure shows that the inhibitor is bound by a Schiff-base link to one of the invariant active-site lysine residues (Lys263). The inhibitor appears to bind in two well defined conformations and the interactions made by it suggest that it is a very close analogue of the substrate 5-aminolaevulinic acid (ALA).

About this Structure

1W31 is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Active as Porphobilinogen synthase, with EC number 4.2.1.24 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Structure of yeast 5-aminolaevulinic acid dehydratase complexed with the inhibitor 5-hydroxylaevulinic acid., Erskine PT, Coates L, Newbold R, Brindley AA, Stauffer F, Beaven GD, Gill R, Coker A, Wood SP, Warren MJ, Shoolingin-Jordan PM, Neier R, Cooper JB, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1222-6. Epub 2005, Aug 16. PMID:16131755

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