1w8a
From Proteopedia
(New page: 200px<br /><applet load="1w8a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w8a, resolution 2.8Å" /> '''THIRD LRR DOMAIN OF D...) |
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| - | [[Image:1w8a.gif|left|200px]]<br /><applet load="1w8a" size=" | + | [[Image:1w8a.gif|left|200px]]<br /><applet load="1w8a" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w8a, resolution 2.8Å" /> | caption="1w8a, resolution 2.8Å" /> | ||
'''THIRD LRR DOMAIN OF DROSOPHILA SLIT'''<br /> | '''THIRD LRR DOMAIN OF DROSOPHILA SLIT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Recognition of the large secreted protein Slit by receptors of the Robo | + | Recognition of the large secreted protein Slit by receptors of the Robo family provides fundamental signals in axon guidance and other developmental processes. In Drosophila, Slit-Robo signalling regulates midline crossing and the lateral position of longitudinal axon tracts. We report the functional dissection of Drosophila Slit, using structure analysis, site-directed mutagenesis and in vitro assays. The N-terminal region of Slit consists of a tandem array of four independently folded leucine-rich repeat (LRR) domains, connected by disulphide-tethered linkers. All three Drosophila Robos were found to compete for a single highly conserved site on the concave face of the second LRR domain of Slit. We also found that this domain is sufficient for biological activity in a chemotaxis assay. Other Slit activities may require Slit dimerisation mediated by the fourth LRR domain. Our results show that a small portion of Slit is able to induce Robo signalling and indicate that the distinct functions of Drosophila Robos are encoded in their divergent cytosolic domains. |
==About this Structure== | ==About this Structure== | ||
| - | 1W8A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1W8A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W8A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Clout, N | + | [[Category: Clout, N J.]] |
[[Category: Hohenester, E.]] | [[Category: Hohenester, E.]] | ||
| - | [[Category: Howitt, J | + | [[Category: Howitt, J A.]] |
[[Category: axon guidance]] | [[Category: axon guidance]] | ||
[[Category: egf-like domain]] | [[Category: egf-like domain]] | ||
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[[Category: signal protein]] | [[Category: signal protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:41:29 2008'' |
Revision as of 13:41, 21 February 2008
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THIRD LRR DOMAIN OF DROSOPHILA SLIT
Overview
Recognition of the large secreted protein Slit by receptors of the Robo family provides fundamental signals in axon guidance and other developmental processes. In Drosophila, Slit-Robo signalling regulates midline crossing and the lateral position of longitudinal axon tracts. We report the functional dissection of Drosophila Slit, using structure analysis, site-directed mutagenesis and in vitro assays. The N-terminal region of Slit consists of a tandem array of four independently folded leucine-rich repeat (LRR) domains, connected by disulphide-tethered linkers. All three Drosophila Robos were found to compete for a single highly conserved site on the concave face of the second LRR domain of Slit. We also found that this domain is sufficient for biological activity in a chemotaxis assay. Other Slit activities may require Slit dimerisation mediated by the fourth LRR domain. Our results show that a small portion of Slit is able to induce Robo signalling and indicate that the distinct functions of Drosophila Robos are encoded in their divergent cytosolic domains.
About this Structure
1W8A is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Binding site for Robo receptors revealed by dissection of the leucine-rich repeat region of Slit., Howitt JA, Clout NJ, Hohenester E, EMBO J. 2004 Nov 10;23(22):4406-12. Epub 2004 Oct 21. PMID:15496984
Page seeded by OCA on Thu Feb 21 15:41:29 2008
