1wbp
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is | + | The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However, the mode of phosphorylation by these kinases and their coordination in the biological regulation of ASF/SF2 is unknown. Here, we report the crystal structure of an active fragment of human SRPK1 bound to a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF/SF2. We find that this docking motif restricts phosphorylation of ASF/SF2 by SRPK1 to the N-terminal part of the RS domain - a property essential for its assembly into nuclear speckles. We further show that Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the C-terminal part of its RS domain. These results suggest that the docking motif of ASF/SF2 is a key regulatory element for sequential phosphorylation by SRPK1 and Clk/Sty and, thus, is essential for its subcellular localization. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Transferred entry: 2 | + | [[Category: Transferred entry: 2 7.11 1]] |
| - | [[Category: Adams, J | + | [[Category: Adams, J A.]] |
| - | [[Category: Aubol, B | + | [[Category: Aubol, B E.]] |
[[Category: Chakrabarti, S.]] | [[Category: Chakrabarti, S.]] | ||
| - | [[Category: Ding, J | + | [[Category: Ding, J H.]] |
| - | [[Category: Fu, X | + | [[Category: Fu, X D.]] |
[[Category: Ghosh, G.]] | [[Category: Ghosh, G.]] | ||
| - | [[Category: Ngo, J | + | [[Category: Ngo, J C.]] |
[[Category: Nolen, B.]] | [[Category: Nolen, B.]] | ||
[[Category: Velazquez-Dones, A.]] | [[Category: Velazquez-Dones, A.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:32 2008'' |
Revision as of 13:42, 21 February 2008
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SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE
Overview
The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However, the mode of phosphorylation by these kinases and their coordination in the biological regulation of ASF/SF2 is unknown. Here, we report the crystal structure of an active fragment of human SRPK1 bound to a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF/SF2. We find that this docking motif restricts phosphorylation of ASF/SF2 by SRPK1 to the N-terminal part of the RS domain - a property essential for its assembly into nuclear speckles. We further show that Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the C-terminal part of its RS domain. These results suggest that the docking motif of ASF/SF2 is a key regulatory element for sequential phosphorylation by SRPK1 and Clk/Sty and, thus, is essential for its subcellular localization.
About this Structure
1WBP is a Protein complex structure of sequences from Homo sapiens with and as ligands. Active as Transferred entry: 2.7.11.1, with EC number 2.7.1.37 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2., Ngo JC, Chakrabarti S, Ding JH, Velazquez-Dones A, Nolen B, Aubol BE, Adams JA, Fu XD, Ghosh G, Mol Cell. 2005 Oct 7;20(1):77-89. PMID:16209947
Page seeded by OCA on Thu Feb 21 15:42:32 2008
Categories: Homo sapiens | Protein complex | Transferred entry: 2 7.11 1 | Adams, J A. | Aubol, B E. | Chakrabarti, S. | Ding, J H. | Fu, X D. | Ghosh, G. | Ngo, J C. | Nolen, B. | Velazquez-Dones, A. | ACT | ADP | Alternative splicing | Atp-binding | Chromosome partition | Differentiation | Kinase | Mrna processing | Mrna splicing | Nuclear protein | Nucleotide-binding | Phosphorylation | Serine/threonine-protein kinase | Srpk | Transferase
