1wcm
From Proteopedia
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==Overview== | ==Overview== | ||
| - | We determined the x-ray structure of the RNA polymerase (Pol) II | + | We determined the x-ray structure of the RNA polymerase (Pol) II subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous structure of the 10-subunit polymerase core, and refined an atomic model of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the complete Pol II structure with structures of the Pol II core and free Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of an alpha-helix in the linker region of the largest Pol II subunit and with folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7 interface explain facilitated Rpb4/7 dissociation in a temperature-sensitive Pol II mutant and specific assembly of Pol I with its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves as the new reference structure for analysis of the transcription mechanism and enables structure solution of complexes of the complete enzyme with additional factors and nucleic acids by molecular replacement. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Armache, K | + | [[Category: Armache, K J.]] |
[[Category: Cramer, P.]] | [[Category: Cramer, P.]] | ||
[[Category: Meinhart, A.]] | [[Category: Meinhart, A.]] | ||
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[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:51 2008'' |
Revision as of 13:42, 21 February 2008
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COMPLETE 12-SUBUNIT RNA POLYMERASE II AT 3.8 ANG
Overview
We determined the x-ray structure of the RNA polymerase (Pol) II subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous structure of the 10-subunit polymerase core, and refined an atomic model of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the complete Pol II structure with structures of the Pol II core and free Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of an alpha-helix in the linker region of the largest Pol II subunit and with folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7 interface explain facilitated Rpb4/7 dissociation in a temperature-sensitive Pol II mutant and specific assembly of Pol I with its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves as the new reference structure for analysis of the transcription mechanism and enables structure solution of complexes of the complete enzyme with additional factors and nucleic acids by molecular replacement.
About this Structure
1WCM is a Protein complex structure of sequences from Saccharomyces cerevisiae with and as ligands. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structures of complete RNA polymerase II and its subcomplex, Rpb4/7., Armache KJ, Mitterweger S, Meinhart A, Cramer P, J Biol Chem. 2005 Feb 25;280(8):7131-4. Epub 2004 Dec 9. PMID:15591044
Page seeded by OCA on Thu Feb 21 15:42:51 2008
Categories: DNA-directed RNA polymerase | Protein complex | Saccharomyces cerevisiae | Armache, K J. | Cramer, P. | Meinhart, A. | Mitterweger, S. | MG | ZN | Cellular rna polymerase | Dna-binding | Dna-dependent rna polymerase | Dna-directed rna polymerase | Metal-binding | Multisubunit co | Nuclear protein | Phosphorylation | Repeat | Transcription | Transferase | Zinc | Zinc-finger
