1wd5
From Proteopedia
(New page: 200px<br /><applet load="1wd5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wd5, resolution 2.00Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1wd5.jpg|left|200px]]<br /><applet load="1wd5" size=" | + | [[Image:1wd5.jpg|left|200px]]<br /><applet load="1wd5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wd5, resolution 2.00Å" /> | caption="1wd5, resolution 2.00Å" /> | ||
'''Crystal structure of TT1426 from Thermus thermophilus HB8'''<br /> | '''Crystal structure of TT1426 from Thermus thermophilus HB8'''<br /> | ||
==Overview== | ==Overview== | ||
| - | TT1426, from Thermus thermophilus HB8, is a conserved hypothetical protein | + | TT1426, from Thermus thermophilus HB8, is a conserved hypothetical protein with a predicted phosphoribosyltransferase (PRTase) domain, as revealed by a Pfam database search. The 2.01 A crystal structure of TT1426 has been determined by the multiwavelength anomalous dispersion (MAD) method. TT1426 comprises a core domain consisting of a central five-stranded beta sheet surrounded by four alpha-helices, and a subdomain in the C terminus. The core domain structure resembles those of the type I PRTase family proteins, although a significant structural difference exists in an inserted 43-residue region. The C-terminal subdomain corresponds to the "hood," which contains a substrate-binding site in the type I PRTases. The hood structure of TT1426 differs from those of the other type I PRTases, suggesting the possibility that TT1426 binds an unknown substrate. The structure-based sequence alignment provides clues about the amino acid residues involved in catalysis and substrate binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1WD5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MES as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1WD5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WD5 OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Kukimoto-Niino, M.]] | [[Category: Kukimoto-Niino, M.]] | ||
[[Category: Murayama, K.]] | [[Category: Murayama, K.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Shibata, R.]] | [[Category: Shibata, R.]] | ||
[[Category: Shirouzu, M.]] | [[Category: Shirouzu, M.]] | ||
| Line 25: | Line 25: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:43:03 2008'' |
Revision as of 13:43, 21 February 2008
|
Crystal structure of TT1426 from Thermus thermophilus HB8
Overview
TT1426, from Thermus thermophilus HB8, is a conserved hypothetical protein with a predicted phosphoribosyltransferase (PRTase) domain, as revealed by a Pfam database search. The 2.01 A crystal structure of TT1426 has been determined by the multiwavelength anomalous dispersion (MAD) method. TT1426 comprises a core domain consisting of a central five-stranded beta sheet surrounded by four alpha-helices, and a subdomain in the C terminus. The core domain structure resembles those of the type I PRTase family proteins, although a significant structural difference exists in an inserted 43-residue region. The C-terminal subdomain corresponds to the "hood," which contains a substrate-binding site in the type I PRTases. The hood structure of TT1426 differs from those of the other type I PRTases, suggesting the possibility that TT1426 binds an unknown substrate. The structure-based sequence alignment provides clues about the amino acid residues involved in catalysis and substrate binding.
About this Structure
1WD5 is a Protein complex structure of sequences from Thermus thermophilus with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a predicted phosphoribosyltransferase (TT1426) from Thermus thermophilus HB8 at 2.01 A resolution., Kukimoto-Niino M, Shibata R, Murayama K, Hamana H, Nishimoto M, Bessho Y, Terada T, Shirouzu M, Kuramitsu S, Yokoyama S, Protein Sci. 2005 Mar;14(3):823-7. Epub 2005 Feb 2. PMID:15689504
Page seeded by OCA on Thu Feb 21 15:43:03 2008
