1wky
From Proteopedia
(New page: 200px<br /><applet load="1wky" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wky, resolution 1.65Å" /> '''Crystal structure of...) |
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| - | [[Image:1wky.gif|left|200px]]<br /><applet load="1wky" size=" | + | [[Image:1wky.gif|left|200px]]<br /><applet load="1wky" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wky, resolution 1.65Å" /> | caption="1wky, resolution 1.65Å" /> | ||
'''Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module'''<br /> | '''Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module'''<br /> | ||
==Overview== | ==Overview== | ||
| - | An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. | + | An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence of the enzyme suggested that the enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method at 277 K. X-ray diffraction data were collected to 1.65 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(-1) and a solvent content of 45.6%. Molecular replacement for initial phasing was carried out using the three-dimensional structure of a mannanase from Thermomonospora fusca as a search model, which corresponds to the catalytic domain of the alkaline mannanase. It gave sufficient phases to build the unknown domain. |
==About this Structure== | ==About this Structure== | ||
| - | 1WKY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.] with CL, CA and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] Full crystallographic information is available from [http:// | + | 1WKY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Akita, M.]] | [[Category: Akita, M.]] | ||
| - | [[Category: Grant, W | + | [[Category: Grant, W D.]] |
[[Category: Hatada, Y.]] | [[Category: Hatada, Y.]] | ||
[[Category: Hirasawa, K.]] | [[Category: Hirasawa, K.]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:45:28 2008'' |
Revision as of 13:45, 21 February 2008
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Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module
Overview
An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence of the enzyme suggested that the enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method at 277 K. X-ray diffraction data were collected to 1.65 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(-1) and a solvent content of 45.6%. Molecular replacement for initial phasing was carried out using the three-dimensional structure of a mannanase from Thermomonospora fusca as a search model, which corresponds to the catalytic domain of the alkaline mannanase. It gave sufficient phases to build the unknown domain.
About this Structure
1WKY is a Protein complex structure of sequences from Bacillus sp. with , and as ligands. Active as Mannan endo-1,4-beta-mannosidase, with EC number 3.2.1.78 Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:15272186
Page seeded by OCA on Thu Feb 21 15:45:28 2008
Categories: Bacillus sp. | Mannan endo-1,4-beta-mannosidase | Protein complex | Akita, M. | Grant, W D. | Hatada, Y. | Hirasawa, K. | Horikoshi, K. | Ito, S. | Kawamoto, M. | Sakai, H. | Takeda, N. | Yamamoto, M. | CA | CL | NA | Catalytic domain | Cbm | Tim barrel
