1wn6

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(New page: 200px<br /><applet load="1wn6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wn6, resolution 1.80&Aring;" /> '''Crystal Structure of...)
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caption="1wn6, resolution 1.80&Aring;" />
'''Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin S'''<br />
'''Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin S'''<br />
==Overview==
==Overview==
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Blasticidin S deaminase from Aspergillus terreus was crystallized with, polyethylene glycol 8000. Two types of crystals were grown under the same, crystallization conditions. One type grew as thin plates, while the other, had a rhombic shape. The rhombic shaped crystal was suitable for, high-resolution crystal structure analysis. Precession photographs and, diffraction data showed that the crystal belonged to orthorhombic space, group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c =, 56.48 A. The calculated Vm value was acceptable when a tetramer of the, enzyme was contained in an asymmetric unit. Preliminary diffraction data, were collected to a resolution of 2.0 A with good statistics.
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Blasticidin S deaminase from Aspergillus terreus was crystallized with polyethylene glycol 8000. Two types of crystals were grown under the same crystallization conditions. One type grew as thin plates, while the other had a rhombic shape. The rhombic shaped crystal was suitable for high-resolution crystal structure analysis. Precession photographs and diffraction data showed that the crystal belonged to orthorhombic space group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c = 56.48 A. The calculated Vm value was acceptable when a tetramer of the enzyme was contained in an asymmetric unit. Preliminary diffraction data were collected to a resolution of 2.0 A with good statistics.
==About this Structure==
==About this Structure==
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1WN6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus] with ZN, BST and ARS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Blasticidin-S_deaminase Blasticidin-S deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.23 3.5.4.23] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WN6 OCA].
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1WN6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=BST:'>BST</scene> and <scene name='pdbligand=ARS:'>ARS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Blasticidin-S_deaminase Blasticidin-S deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.23 3.5.4.23] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WN6 OCA].
==Reference==
==Reference==
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[[Category: zinc]]
[[Category: zinc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:32:31 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:46:07 2008''

Revision as of 13:46, 21 February 2008

Image:1wn6.gif

1wn6, resolution 1.80Å

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Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin S

Overview

Blasticidin S deaminase from Aspergillus terreus was crystallized with polyethylene glycol 8000. Two types of crystals were grown under the same crystallization conditions. One type grew as thin plates, while the other had a rhombic shape. The rhombic shaped crystal was suitable for high-resolution crystal structure analysis. Precession photographs and diffraction data showed that the crystal belonged to orthorhombic space group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c = 56.48 A. The calculated Vm value was acceptable when a tetramer of the enzyme was contained in an asymmetric unit. Preliminary diffraction data were collected to a resolution of 2.0 A with good statistics.

About this Structure

1WN6 is a Single protein structure of sequence from Aspergillus terreus with , and as ligands. Active as Blasticidin-S deaminase, with EC number 3.5.4.23 Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:10089374

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