1woq
From Proteopedia
(New page: 200px<br /><applet load="1woq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1woq, resolution 1.80Å" /> '''Crystal Structure of...) |
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| - | [[Image:1woq.jpg|left|200px]]<br /><applet load="1woq" size=" | + | [[Image:1woq.jpg|left|200px]]<br /><applet load="1woq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1woq, resolution 1.80Å" /> | caption="1woq, resolution 1.80Å" /> | ||
'''Crystal Structure of Inorganic Polyphosphate/ATP-Glucomannokinase From Arthrobacter sp. strain KM At 1.8 A Resolution'''<br /> | '''Crystal Structure of Inorganic Polyphosphate/ATP-Glucomannokinase From Arthrobacter sp. strain KM At 1.8 A Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Inorganic polyphosphate (poly(P)) is a biological high energy compound | + | Inorganic polyphosphate (poly(P)) is a biological high energy compound presumed to be an ancient energy carrier preceding ATP. Several poly(P)-dependent kinases that use poly(P) as a phosphoryl donor are known to function in bacteria, but crystal structures of these kinases have not been solved. Here we present the crystal structure of bacterial poly(P)/ATP-glucomannokinase, belonging to Gram-positive bacterial glucokinase, complexed with 1 glucose molecule and 2 phosphate molecules at 1.8 A resolution, being the first among poly(P)-dependent kinases and bacterial glucokinases. The poly(P)/ATP-glucomannokinase structure enabled us to understand the structural relationship of bacterial glucokinase to eucaryotic hexokinase and ADP-glucokinase, which has remained a matter of debate. These comparisons also enabled us to propose putative binding sites for phosphoryl groups for ATP and especially for poly(P) and to obtain insights into the evolution of kinase, particularly from primordial poly(P)-specific to ubiquitous ATP-specific proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1WOQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_sp. Arthrobacter sp.] with GLC and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polyphosphate--glucose_phosphotransferase Polyphosphate--glucose phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.63 2.7.1.63] Full crystallographic information is available from [http:// | + | 1WOQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_sp. Arthrobacter sp.] with <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polyphosphate--glucose_phosphotransferase Polyphosphate--glucose phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.63 2.7.1.63] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WOQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: polyphosphate]] | [[Category: polyphosphate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:46:46 2008'' |
Revision as of 13:46, 21 February 2008
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Crystal Structure of Inorganic Polyphosphate/ATP-Glucomannokinase From Arthrobacter sp. strain KM At 1.8 A Resolution
Overview
Inorganic polyphosphate (poly(P)) is a biological high energy compound presumed to be an ancient energy carrier preceding ATP. Several poly(P)-dependent kinases that use poly(P) as a phosphoryl donor are known to function in bacteria, but crystal structures of these kinases have not been solved. Here we present the crystal structure of bacterial poly(P)/ATP-glucomannokinase, belonging to Gram-positive bacterial glucokinase, complexed with 1 glucose molecule and 2 phosphate molecules at 1.8 A resolution, being the first among poly(P)-dependent kinases and bacterial glucokinases. The poly(P)/ATP-glucomannokinase structure enabled us to understand the structural relationship of bacterial glucokinase to eucaryotic hexokinase and ADP-glucokinase, which has remained a matter of debate. These comparisons also enabled us to propose putative binding sites for phosphoryl groups for ATP and especially for poly(P) and to obtain insights into the evolution of kinase, particularly from primordial poly(P)-specific to ubiquitous ATP-specific proteins.
About this Structure
1WOQ is a Single protein structure of sequence from Arthrobacter sp. with and as ligands. Active as Polyphosphate--glucose phosphotransferase, with EC number 2.7.1.63 Full crystallographic information is available from OCA.
Reference
Crystal structure of bacterial inorganic polyphosphate/ATP-glucomannokinase. Insights into kinase evolution., Mukai T, Kawai S, Mori S, Mikami B, Murata K, J Biol Chem. 2004 Nov 26;279(48):50591-600. Epub 2004 Sep 17. PMID:15377666
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